ATP6V1B2 Antikörper

Produktnummer ABIN1573989

Produktdetails anti-ATP6V1B2 Antikörper

Target: ATP6V1B2 (ATPase, H+ Transporting, Lysosomal 56/58kDa, V1 Subunit B2 (ATP6V1B2))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Huhn, Drosophila melanogaster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATP6V1B2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Spezifität: Rabbit Anti-V-ATPase Subunit B 2 Polyclonal Antibody reacts with human Anti-V-ATPase subunit B. Sequence homology predicts that it will also react with mouse, rat, chicken, bovine, and fruit fly V-ATPase subunit B 2.

Kreuzreaktivität (Details): Rabbit Anti-V-ATPase Subunit B 2 Polyclonal Antibody reacts with human Anti-V-ATPase subunit B. Sequence homology predicts that it will also react with mouse, rat, chicken, bovine, and fruit fly V-ATPase subunit B 2.

Aufreinigung: Immunoaffinity chromatography

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.03-0.125 µg/mL Western blot: 1.0 µg/mL Other applications: user-optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS, pH 7.4, containing 30 % glycerol and 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freezing and thawing cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The antibody is stable for 2-3 weeks if stored at 2-8 °C. For long term storage, aliquot and store at -28 °C or below.

Referenzen für anti-ATP6V1B2 Antikörper (ABIN1573989)

Ahmad, Khundmiri, Pribble, Merchant, Ameen, Klein, Levi, Lederer: "Role of vacuolar ATPase in the trafficking of renal type IIa sodium-phosphate cotransporter." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 27, Issue 6, pp. 703-14, (2011) (PubMed).

Details zu ATP6V1B2

Target: ATP6V1B2 (ATPase, H+ Transporting, Lysosomal 56/58kDa, V1 Subunit B2 (ATP6V1B2))

Andere Bezeichnung: V-ATPase Subunit B 2 (ATP6V1B2 Produkte)

Synonyme: Atp6b1b2 antikoerper, Atp6b2 antikoerper, Vatb antikoerper, vha55 antikoerper, VATB antikoerper, ATP6B1B2 antikoerper, ATP6B2 antikoerper, HO57 antikoerper, VPP3 antikoerper, Vma2 antikoerper, atp6v1bb antikoerper, fj51e01 antikoerper, vatB2 antikoerper, wu:fj51e01 antikoerper, zgc:109771 antikoerper, AI194269 antikoerper, AI790362 antikoerper, R74844 antikoerper, ATPase H+ transporting V1 subunit B2 antikoerper, ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2 S homeolog antikoerper, ATPase, H+ transporting, lysosomal, V1 subunit B2 antikoerper, ATPase, H+ transporting, lysosomal V1 subunit B2 antikoerper, Atp6v1b2 antikoerper, atp6v1b2.S antikoerper, ATP6V1B2 antikoerper, atp6v1b2 antikoerper

Hintergrund: The V-ATPase subunit B 2 is related to a gene that encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of two V1 domain B subunit isoforms and is the only B isoform highly expressed in osteoclasts.Rabbit Anti-V-ATPase Subunit B 2 Polyclonal Antibody is developed in rabbit using a synthetic peptide conjugated to KLH. It is purified from rabbit antiserum by immunoaffinity chromatography and supplied as 40 ug aliquot at concentration of 0.5 mg/ml.

Pathways: Transition Metal Ion Homeostasis, Proton Transport