ATP6V1A Antikörper

Produktnummer ABIN1573979

Produktdetails anti-ATP6V1A Antikörper

Target: ATP6V1A (ATPase, H+ Transporting, Lysosomal 70kDa, V1 Subunit A (ATP6V1A))

Reaktivität: Human, Maus, Rind (Kuh), Manduca, Nicotiana tabacum, Huhn, Orang-Utan, Drosophila melanogaster, Fisch

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATP6V1A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Spezifität: Rabbit Anti-V-ATPase Subunit A Polyclonal Antibody reacts with mouse V-ATPase subunit A. Sequence homology predicts that it will also react with human, bovine, orangutan, tobacco hornworm, chicken, fruit fly, and zebrafish V-ATPase subunit A.

Kreuzreaktivität (Details): Rabbit Anti-V-ATPase Subunit A Polyclonal Antibody reacts with mouse V-ATPase subunit A. Sequence homology predicts that it will also react with human, bovine, orangutan, tobacco hornworm, chicken, fruit fly, and zebrafish V-ATPase subunit A.

Aufreinigung: Immunoaffinity chromatography

Immunogen: Synthetic peptide conjugated - KLH

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.02-0.2 µg/mL
Western blot: 1.0 µg/mL
Other applications: user-optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS, pH 7.4, containing 30 % glycerol, and 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freezing and thawing cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The antibody is stable for 2-3 weeks if stored at 2-8 °C. For long term storage, aliquot and store at -28 °C or below.

Referenzen für anti-ATP6V1A Antikörper (ABIN1573979)

Gokey, Dasgupta, Amack: "The V-ATPase accessory protein Atp6ap1b mediates dorsal forerunner cell proliferation and left-right asymmetry in zebrafish." in: Developmental biology, Vol. 407, Issue 1, pp. 115-30, (2016) (PubMed).

Grubman, Lidgerwood, Duncan, Bica, Tan, Parker, Caragounis, Meyerowitz, Volitakis, Moujalled, Liddell, Hickey, Horne, Longmuir, Koistinaho, Donnelly, Crouch, Tammen, White, Kanninen: "Deregulation of subcellular biometal homeostasis through loss of the metal transporter, Zip7, in a childhood neurodegenerative disorder." in: Acta neuropathologica communications, Vol. 2, pp. 25, (2014) (PubMed).

Monteiro, Aires, Becker, Jacinto, Certal, Rodríguez-León: "V-ATPase proton pumping activity is required for adult zebrafish appendage regeneration." in: PLoS ONE, Vol. 9, Issue 3, pp. e92594, (2014) (PubMed).

Li, Chen, Klemmer, Koopmans, Karupothula, Smit: "Identifying true protein complex constituents in interaction proteomics: the example of the DMXL2 protein complex." in: Proteomics, Vol. 12, Issue 15-16, pp. 2428-32, (2012) (PubMed).

Nylandsted, Becker, Bunkenborg, Andersen, Dengjel, Jäättelä: "ErbB2-associated changes in the lysosomal proteome." in: Proteomics, Vol. 11, Issue 14, pp. 2830-8, (2011) (PubMed).

Details zu ATP6V1A

Target: ATP6V1A (ATPase, H+ Transporting, Lysosomal 70kDa, V1 Subunit A (ATP6V1A))

Andere Bezeichnung: V-ATPase Subunit A (ATP6V1A Produkte)

Synonyme: ATP6A1 antikoerper, ATP6V1A1 antikoerper, HO68 antikoerper, VA68 antikoerper, VPP2 antikoerper, Vma1 antikoerper, AI647066 antikoerper, Atp6a1 antikoerper, Atp6a2 antikoerper, Atp6v1a1 antikoerper, ATP6V1A antikoerper, atp6v1al antikoerper, zgc:63516 antikoerper, An02g10440 antikoerper, AO090102000349 antikoerper, vacuolar ATP synthase subunit A antikoerper, atp6a1 antikoerper, atp6v1a1 antikoerper, ho68 antikoerper, v1a antikoerper, va68 antikoerper, vma1 antikoerper, vpp2 antikoerper, CG12403 antikoerper, Dmel\CG12403 antikoerper, V-ATPase antikoerper, Vha antikoerper, Vha-68-1 antikoerper, Vha68 antikoerper, vha67-2 antikoerper, vha68-1 antikoerper, ATPase H+ transporting V1 subunit A antikoerper, ATPase, H+ transporting, lysosomal V1 subunit A antikoerper, ATPase, H+ transporting, lysosomal, V1 subunit Aa antikoerper, v-type proton ATPase catalytic subunit A antikoerper, vacuolar ATP synthase subunit A antikoerper, ATPase H+ transporting V1 subunit A L homeolog antikoerper, vacuolar proton pump 3 antikoerper, Vacuolar H[+] ATPase 68kD subunit 1 antikoerper, ATP6V1A antikoerper, Atp6v1a antikoerper, atp6v1aa antikoerper, ANI_1_1468024 antikoerper, AOR_1_602134 antikoerper, VHA-A antikoerper, atp6v1a.L antikoerper, vpp3 antikoerper, Vha68-1 antikoerper

Hintergrund: The V-ATPase subunit A is a catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex attached to an integral membrane V0 proton pore complex.Rabbit Anti-V-ATPase Subunit A Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide, purified from rabbit antiserum by immunoaffinity chromatography, and supplied as 40 ug aliquot at concentration of 0.5 mg/ml.

Pathways: Transition Metal Ion Homeostasis, Proton Transport, SARS-CoV-2 Protein Interaktom