V5 Epitope Tag Antikörper (HRP)

Details zu Produkt Nr. ABIN1573947, Anbieter: Anmelden zum Anzeigen
Antigen
Reaktivität
Tag
401
18
16
3
3
Wirt
Kaninchen
248
135
36
7
5
4
3
1
1
Klonalität
Polyklonal
Konjugat
HRP
30
28
21
13
12
12
11
11
11
10
10
10
10
9
8
8
7
6
5
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Applikation
ELISA, Western Blotting (WB)
366
261
137
94
77
71
52
36
28
13
9
8
5
4
4
2
2
2
1
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
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Immunogen V5 epitope tag peptide GKPIPNPLLGLDST conjugated - KLH
Sequenz GKPIPNPLLG LDST
Isotyp IgG
Spezifität Rabbit Anti-V5-tag [HRP] Polyclonal Antibody specifically recognizes C-terminal, N-terminal, and internal V5 tagged fusion proteins.
Reinigung Immunoaffinity chromatography
Andere Bezeichnung V5-Tag
Substanzklasse Tag
Hintergrund Rabbit Anti-V5-tag [HRP] Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. Rabbit Anti-V5-tag [HRP] polyclonal antibody is suitable for detecting fusion proteins that contain V5-tags.
Forschungsgebiet Tags/Labels
Applikationshinweise Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mL
Other applications: user-optimized
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer PBS, pH 7.4, containing 1 % BSA and 0.01 % thimerosal
Konservierungsmittel Thimerosal (Merthiolate)
Vorsichtsmaßnahmen This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Bilder des Herstellers
Western Blotting (WB) image for anti-V5 Epitope Tag antibody (HRP) (ABIN1573947) Lane 1-2: V5-tag fusion protein in Hela cell lysate (~52 kD) Lane 3: Negative Hela ce...
Produkt verwendet in: Dahl, Corydon, Ränkel, Nielsen, Füchtbauer, Knudsen: "An eEF1A1 truncation encoded by PTI-1 exerts its oncogenic effect inside the nucleus." in: Cancer cell international, Vol. 14, Issue 1, pp. 17, 2014 (PubMed).

Tahir, Yang, Goltsov, Song, Ren, Wang, Chang, Thompson: "Caveolin-1-LRP6 signaling module stimulates aerobic glycolysis in prostate cancer." in: Cancer research, Vol. 73, Issue 6, pp. 1900-11, 2013 (PubMed).

Zhang, Li, Jaiswal, Bayat, Xiong, Sandoval, Charng, David, Haueter, Yamamoto, Graham, Bellen: "The C8ORF38 homologue Sicily is a cytosolic chaperone for a mitochondrial complex I subunit." in: The Journal of cell biology, Vol. 200, Issue 6, pp. 807-20, 2013 (PubMed).

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