Integrin alpha 4 (ITGA4) Antikörper

Antigen: Integrin alpha 4 (ITGA4)

Synonyme: CD49D, IA4, MGC90518, LOC397839, ITGA4

Klonalität: Monoklonal (MRa4-1-MRalpha4-1)

Wirt: Maus

Reaktivität: Ratte (Rattus)

Applikation: Durchflusszytometrie (FACS), Immunpräzipitation (IP), Aktivierung (Act), Blockierende Antikörper (Inhibition)

Produktnummer: ABIN157242

Produktbeschreibung

Weitere Bezeichnung: CD49d

Immunogen: RBL-2H3 rat basophilic leukemia cell line

Format: Low endotoxin (sterile), Low-endotoxin, azide-free (LEAF)

Isotyp: IgG2a, kappa chain  (Passende Sekundärantikörper)

Klon: MRa4-1-MRalpha4-1

Beschreibung: CD49d is a 150 kD glycoprotein, also known as integrin alpha4 or VLA-4 alpha chain. It is expressed as a heterodimer with either of two beta chains, beta1 or beta7. The alpha4beta1 integrin (also known as VLA-4, CD49d/CD29) is expressed on peripheral T and B lymphocytes, thymocytes, and monocytes, while the alpha4beta7 integrin (also known as LPAM-1) is expressed on peripheral lymphocytes. The alpha4beta1 and alpha4beta7 integrins mediate cell-cell and cell-matrix interactions by interacting with the ligands CD106 (VCAM-1), MAdCAM-1, and fibronectin. Soluble MRalpha4-1 antibody can partially inhibit in vitro binding of VCAM-1 and mast cells to fibronectin and inhibit mast cell degranulation. Immobilized MRalpha4-1 antibody can enhance mast cell degranulation in vitro. Distribution: alpha4/beta1- peripheral T and B lymphocytes, thymocytes, and monocytes, alpha4/beta7- peripheral lymphocytes Function: Adhesion Synonyms: Integrin alpha4 chain, alpha4 integrin, VLA-4 alpha chain Structure: Integrin alpha chain, 150 kD

Anwendungen

Applikationshinweise: Additional reported applications include: immunoprecipitation, in vitro activation, in vitro inhibition of binding of VCAM-1 and mast cells to fibronectin , and in vivo inhibition of VLA-4 functions. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN157242). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Reinheit: LEAF

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.

Lagerung: The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.

Forschungsgebiet: CD Antigene

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Lou rat blood lymphocytes stained with MRalpha4-1 FITC

Publikationen

Publikationen: Issekutz, Wykretowicz: "Effect of a new monoclonal antibody, TA-2, that inhibits lymphocyte adherence to cytokine stimulated endothelium in the rat." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 1, pp. 109-16, 1991 (PubMed).

Issekutz: "Inhibition of in vivo lymphocyte migration to inflammation and homing to lymphoid tissues by the TA-2 monoclonal antibody. A likely role for VLA-4 in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 12, pp. 4178-84, 1992 (PubMed).

Springer: "Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm." in: Cell, Vol. 76, Issue 2, pp. 301-14, 1994 (PubMed).

Yang, Issekutz, Wright: "Prolongation of rat islet allograft survival by treatment with monoclonal antibodies against VLA-4 and LFA-1." in: Transplantation, Vol. 60, Issue 1, pp. 71-6, 1995 (PubMed).

Yasuda, Hasunuma, Adachi et al.: "Expression and function of fibronectin binding integrins on rat mast cells." in: International immunology, Vol. 7, Issue 2, pp. 251-8, 1995 (PubMed).

Palecanda, Briskin, Issekutz: "Rat mast cell lines bind to the vascular cell adhesion molecule-1 (VCAM-1) and the mucosal addressin cell adhesion molecule-1 (MAdCAM-1)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 6, pp. 2904-10, 1997 (PubMed).