CD28 (CD28) Antikörper

Antigen: CD28 (CD28)

Synonyme: Tp44, MGC138290

Klonalität: Monoklonal (E18)

Wirt: Maus

Reaktivität: Maus

Applikation: Durchflusszytometrie (FACS), Kostimulation

Produktnummer: ABIN157184

Produktbeschreibung

Weitere Bezeichnung: CD28

Immunogen: A20 cells and recombinant mCD28Ig

Format: Low endotoxin (sterile), Low-endotoxin, azide-free (LEAF)

Isotyp: IgG2b, kappa chain  (Passende Sekundärantikörper)

Klon: E18

Beschreibung: CD28 is a 44 kD glycoprotein, also known as Tp44 or T44. It is a member of the Ig superfamily, expressed on thymocytes, most peripheral T cells, and NK cells. In association with CD80 (B7-1) and CD86 (B7-2), CD28 acts as the second signal for T and NK cell activation and proliferation. The E18 antibody blocks the binding of 37.51 antibody to CD28 and costimulates T cell activation and proliferation. Distribution: Thymocytes, CD4, CD8 peripheral T cells, NK cells Function: Costimulates T and NK cells Synonyms: Tp44, T44 Structure: Ig superfamily, 44 kD

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: in vitro costimulation of T and NK cells1, in vitro blocking of allogeneic mixed leukocyte response and inhibition of MHC-unrestricted CTL cytotoxicity, and in vitro induction of thymocyte differentiation. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN157184). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 1.0 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Reinheit: LEAF

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.

Lagerung: The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.

Forschungsgebiet: CD Antigene

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-CD28 (CD28) antibody

Publikationen

Publikationen: Gross, Michl: "Re: Functional characteristics of sperm obtained by electroejaculation." in: The Journal of urology, Vol. 149, Issue 2, pp. 380, 1993 (PubMed).

Lenschow, Walunas, Bluestone: "CD28/B7 system of T cell costimulation." in: Annual review of immunology, Vol. 14, pp. 233-58, 1996 (PubMed).

Dennehy, Elias, Zeder-Lutz et al.: "Cutting edge: monovalency of CD28 maintains the antigen dependence of T cell costimulatory responses." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 10, pp. 5725-9, 2006 (PubMed).