Qa-2 Antikörper (Alexa Fluor 647)

Antigen: Qa-2

Klonalität: Monoklonal (695H1-9-9)

Wirt: Maus

Reaktivität: Maus

Konjugat: Alexa Fluor 647

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN157160

Produktbeschreibung

Immunogen: C3H.SW mouse skin graft and splenocytes

Isotyp: IgG2a, kappa chain

Klon: 695H1-9-9

Beschreibung: Mouse Qa-2 is a 40kD GPI-linked surface protein. It is a member of nonclassical MHC class Ib molecule encoded by Q5, Q6, Q7, Q9, and Q10 genes of H-2 complex. Qa-2 is a nonpolymorphic molecule primarily expressed on mouse T and B cells. The expression level of Qa-2 on mature T cells is higher than B cells. Different mouse strains are with various level of Qa-2 expression. Qa-2, in association with beta2-microglobulin, binds to peptide and plays a role in antigen presentation. Distribution: T and B cells, mature T cells expression higher than B cells Function: Antigen presentation, promote immune response Synonyms: Nonclassical MHC-Ib, H2-Qa2 Structure: GPI-linked protein, associate with ß2-microglobulin, 40kD

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: immunoprecipitation. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.

Reinigung: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Lagerung: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

C57BL/6 mouse splenocytes stained with 695H1-9-9 Alexa Fluor(R) 647

Publikationen

Publikationen: Sharrow, Arn, Stroynowski et al.: "Epitope clusters of Qa-2 antigens defined by a panel of new monoclonal antibodies." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 10, pp. 3495-502, 1989 (PubMed).

Mann, Stroynowski, Hood et al.: "Cytotoxic T lymphocytes from mice with soluble class I Q10 molecules in their serum are not tolerant to membrane-bound Q10." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 1, pp. 240-5, 1987 (PubMed).

Mellor, Antoniou, Robinson: "Structure and expression of genes encoding murine Qa-2 class I antigens." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 82, Issue 17, pp. 5920-4, 1985 (PubMed).

Tabaczewski, Chiang, Henson et al.: "Alternative peptide binding motifs of Qa-2 class Ib molecules define rules for binding of self and nonself peptides." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 6, pp. 2771-81, 1997 (PubMed).

Chiang, Stroynowski: "Protective immunity against disparate tumors is mediated by a nonpolymorphic MHC class I molecule." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 9, pp. 5367-74, 2005 (PubMed).