Qa-2 Antikörper

Antigen: Qa-2

Klonalität: Monoklonal (695H1-9-9)

Wirt: Maus

Reaktivität: Maus

Applikation: Durchflusszytometrie (FACS), Immunpräzipitation (IP)

Produktnummer: ABIN157154

Produktbeschreibung

Immunogen: C3H.SW mouse skin graft and splenocytes

Format: Purified

Isotyp: IgG2a, kappa chain  (Passende Sekundärantikörper)

Klon: 695H1-9-9

Beschreibung: Mouse Qa-2 is a 40kD GPI-linked surface protein. It is a member of nonclassical MHC class Ib molecule encoded by Q5, Q6, Q7, Q9, and Q10 genes of H-2 complex. Qa-2 is a nonpolymorphic molecule primarily expressed on mouse T and B cells. The expression level of Qa-2 on mature T cells is higher than B cells. Different mouse strains are with various level of Qa-2 expression. Qa-2, in association with beta2-microglobulin, binds to peptide and plays a role in antigen presentation. Distribution: T and B cells, mature T cells expression higher than B cells Function: Antigen presentation, promote immune response Synonyms: Nonclassical MHC-Ib, H2-Qa2 Structure: GPI-linked protein, associate with beta2-microglobulin, 40kD

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: immunoprecipitation. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Lagerung: The antibody solution should be stored undiluted at 4°C.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-Qa-2 antibody

Publikationen

Publikationen: Sharrow, Arn, Stroynowski et al.: "Epitope clusters of Qa-2 antigens defined by a panel of new monoclonal antibodies." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 10, pp. 3495-502, 1989 (PubMed).

Mann, Stroynowski, Hood et al.: "Cytotoxic T lymphocytes from mice with soluble class I Q10 molecules in their serum are not tolerant to membrane-bound Q10." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 1, pp. 240-5, 1987 (PubMed).

Mellor, Antoniou, Robinson: "Structure and expression of genes encoding murine Qa-2 class I antigens." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 82, Issue 17, pp. 5920-4, 1985 (PubMed).

Tabaczewski, Chiang, Henson et al.: "Alternative peptide binding motifs of Qa-2 class Ib molecules define rules for binding of self and nonself peptides." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 6, pp. 2771-81, 1997 (PubMed).

Chiang, Stroynowski: "Protective immunity against disparate tumors is mediated by a nonpolymorphic MHC class I molecule." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 9, pp. 5367-74, 2005 (PubMed).