CD180 Molecule (CD180) Antikörper

Antigen: CD180 Molecule (CD180)

Synonyme: LY64, Ly78, RP105, MGC126233, MGC126234, Lrrp, Ly64, NJ-1, 14/A10, 114/A10, AI159736, CD180, Gir, RP39, RP82, Gpr72, AW045253, F630107B15

Klonalität: Monoklonal (RP-14)

Wirt: Ratte

Reaktivität: Maus

Applikation: Durchflusszytometrie (FACS), Immunpräzipitation (IP), Aktivierung (Act), Apoptose-Induktion (AI)

Produktnummer: ABIN156941

Produktbeschreibung

Weitere Bezeichnung: CD180 (RP105)

Immunogen: BALB/c mouse splenocytes

Format: Low endotoxin (sterile), Low-endotoxin, azide-free (LEAF)

Isotyp: IgG2a, kappa chain  (Passende Sekundärantikörper)

Klon: RP-14

Beschreibung: CD180 is a 105 kD type I membrane protein, also known as RP105 or Ly64. It is a member of the toll-like receptor family consisting of an extracellular leucine-rich repeat (LRR) and a short cytoplasmic tail. It is expressed on most B cells, monocytes/macrophages, and dendritic cells. CD180 associates with MD-1 to form the RP105/MD-1 surface receptor. CD180/MD-1, by working in concert with TLR4, controls B cell recognition and signalling of bacterial LPS. The ligation of RP105 by RP/14 leads to a strong B cell proliferative response, as well as protection from apoptosis induced by radiation or dexamethasone. Interestingly, in some systems, ligation of RP105 leads to Fas-independent apoptosis. Distribution: B cells, monocytes/macrophages, and dendritic cells Function: Signaling protein involved in innate immunity Synonyms: CD180, Radio-protective 105-kD protein, RP105 Structure: Member of the toll-like receptor family, 105 kD

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: immunoprecipitation, B cell activation, protection of B cells from apoptosis induced by radiation or dexamethasone and induction of Fas-independent apoptosis. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN156941). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 1.0 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Reinheit: LEAF

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.

Lagerung: The antibody solution should be stored undiluted at 4°C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.

Forschungsgebiet: CD Antigene

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-CD180 Molecule (CD180) antibody

Publikationen

Publikationen: Miyake, Yamashita, Hitoshi et al.: "Murine B cell proliferation and protection from apoptosis with an antibody against a 105-kD molecule: unresponsiveness of X-linked immunodeficient B cells." in: The Journal of experimental medicine, Vol. 180, Issue 4, pp. 1217-24, 1994 (PubMed).

Miyake, Yamashita, Ogata et al.: "RP105, a novel B cell surface molecule implicated in B cell activation, is a member of the leucine-rich repeat protein family." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 7, pp. 3333-40, 1995 (PubMed).

Yamashita, Miyake, Miura et al.: "Activation mediated by RP105 but not CD40 makes normal B cells susceptible to anti-IgM-induced apoptosis: a role for Fc receptor coligation." in: The Journal of experimental medicine, Vol. 184, Issue 1, pp. 113-20, 1996 (PubMed).

Chan, Mecklenbraeuker, Su et al.: "The molecular mechanism of B cell activation by toll-like receptor protein RP-105." in: The Journal of experimental medicine, Vol. 188, Issue 1, pp. 93-101, 1998 (PubMed).

Miyake, Shimazu, Kondo et al.: "Mouse MD-1, a molecule that is physically associated with RP105 and positively regulates its expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 3, pp. 1348-53, 1998 (PubMed).