Killer Cell Lectin-Like Receptor, Subfamily A, Member 1 (KLRA1) Antikörper

Antigen: Killer Cell Lectin-Like Receptor, Subfamily A, Member 1 (KLRA1)

Synonyme: A1, Ly49a, Ly49v, Klra22, Ly49o<129>

Klonalität: Monoklonal (YE1-48-10-6)

Wirt: Ratte

Reaktivität: Maus

Applikation: Durchflusszytometrie (FACS), Immunpräzipitation (IP)

Produktnummer: ABIN156893

Produktbeschreibung

Weitere Bezeichnung: Ly-49A

Immunogen: Con A activated splenic T cells

Format: Purified

Isotyp: IgG2a, kappa chain  (Passende Sekundärantikörper)

Klon: YE1-48-10-6

Beschreibung: Ly-49A is a 44 kD member of the C-type lectin/Ly-49 family proteins. It is a type-II transmembrane, disulfide-linked homodimer with C-type lectin extracellular domains. Ly-49A is present on a subset of NK and NK-T cells. Ly-49A, like other members of this family, inhibits NK cell activation through the recognition and interaction of certain MHC class I haplotypes on potential target cells. The Ly-49A receptor recognizes H-2D and H-2D, triggering phosphorylation of the ITIM motif in the cytoplasmic domain and recruitment of SHP-1 and SHP-2. The YE1/48.10.6 antibody reacts with NK cells from all mouse strains tested and reverses the inhibition of NK cytotoxicity. Distribution: NK and NK-T cells Function: Inhibitory NK cell receptor Structure: C-type lectin, Ly-49, ITIM, 44 kD

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: immunoprecipitation. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Lagerung: The antibody solution should be stored undiluted at 4°C.

Forschungsgebiet: Zell-/Gewebemarker, Immunologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

C57BL/6 mouse splenocytes stained with YE1/48.10.6 PE and DX5 FITC

Publikationen

Publikationen: Chan, Takei: "Molecular cloning and characterization of a novel murine T cell surface antigen, YE1/48." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 5, pp. 1727-36, 1989 (PubMed).

Takei: "Two surface antigens expressed on proliferating mouse T lymphocytes defined by rat monoclonal antibodies." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 130, Issue 6, pp. 2794-7, 1983 (PubMed).

Takei, Brennan, Mager: "The Ly-49 family: genes, proteins and recognition of class I MHC." in: Immunological reviews, Vol. 155, pp. 67-77, 1997 (PubMed).

Hanke, Takizawa, McMahon et al.: "Direct assessment of MHC class I binding by seven Ly49 inhibitory NK cell receptors." in: Immunity, Vol. 11, Issue 1, pp. 67-77, 1999 (PubMed).

Coles, McMahon, Takizawa et al.: "Memory CD8 T lymphocytes express inhibitory MHC-specific Ly49 receptors." in: European journal of immunology, Vol. 30, Issue 1, pp. 236-44, 2000 (PubMed).