H-2Kd Antikörper (Alexa Fluor 647)

Antigen: H-2Kd

Klonalität: Monoklonal (SF1-1-1)

Wirt: Maus

Reaktivität: Maus

Konjugat: Alexa Fluor 647

Applikation: Durchflusszytometrie (FACS), Immunhistochemie (Gefrierschnitte) (IHC (fro)), Immunfluoreszenz (IF)

Produktnummer: ABIN156889

Produktbeschreibung

Immunogen: BALB/c Mouse cells

Isotyp: IgG2a, IgG2a, kappa chain

Klon: SF1-1-1

Beschreibung: The SF1-1.1 antibody reacts with the H-2K MHC class I alloantigens expressed on nucleated cells from mice of the H-2K haplotype. H-2K is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The SF1-1.1 antibody is weakly cross-reactive with H-2, but does not cross-react with other haplotypes (b, j, p, q, s, v). Distribution: Nucleated cells of the H-2K haplotype Function: Antigen presentation Synonyms: MHC class I Structure: MHC class I

Spezifität: Weakly cross-reacts with h-2 haplotype

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting, and immunohistochemical staining of acetone-fixed frozen sections. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.

Reinigung: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Lagerung: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

BALB/c mouse splenocytes stained with SF1-1.1 Alexa Fluor(R) 647

Publikationen

Publikationen: York, Rock: "Antigen processing and presentation by the class I major histocompatibility complex." in: Annual review of immunology, Vol. 14, pp. 369-96, 1996 (PubMed).

Noun, Reboul, Abastado et al.: "Alloreactive monoclonal antibodies select Kd molecules with different peptide profiles." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 6, pp. 2455-61, 1996 (PubMed).

Bashuda, Seino, Ra et al.: "Lack of cognate help by CD4+ T cells and anergy of CD8+ T cells are the principal mechanisms for anti-leukocyte function-associated antigen-1/intercellular adhesion molecule-1-induced cardiac allograft tolerance." in: Transplantation, Vol. 63, Issue 1, pp. 113-8, 1997 (PubMed).

Watts: "Capture and processing of exogenous antigens for presentation on MHC molecules." in: Annual review of immunology, Vol. 15, pp. 821-50, 1997 (PubMed).

Pamer, Cresswell: "Mechanisms of MHC class I--restricted antigen processing." in: Annual review of immunology, Vol. 16, pp. 323-58, 1998 (PubMed).

Norian, Allen: "No intrinsic deficiencies in CD8+ T cell-mediated antitumor immunity with aging." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 2, pp. 835-44, 2004 (PubMed).

Ma, Xu, An et al.: "Vaccine with beta-defensin 2-transduced leukemic cells activates innate and adaptive immunity to elicit potent antileukemia responses." in: Cancer research, Vol. 66, Issue 2, pp. 1169-76, 2006 (PubMed).