H-2Kd Antikörper (FITC)

Antigen: H-2Kd

Klonalität: Monoklonal (SF1-1-1)

Wirt: Maus

Reaktivität: Maus

Konjugat: FITC

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN156883

Produktbeschreibung

Immunogen: BALB/c Mouse cells

Isotyp: IgG2a, IgG2a, kappa chain

Klon: SF1-1-1

Beschreibung: The SF1-1.1 antibody reacts with the H-2K MHC class I alloantigens expressed on nucleated cells from mice of the H-2K haplotype. H-2K is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The SF1-1.1 antibody is weakly cross-reactive with H-2, but does not cross-react with other haplotypes (b, j, p, q, s, v). Distribution: Nucleated cells of the H-2K haplotype Function: Antigen presentation Synonyms: MHC class I Structure: MHC class I

Spezifität: Weakly cross-reacts with h-2 haplotype

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting, and immunohistochemical staining of acetone-fixed frozen sections. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. Product Information

Reinigung: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Lagerung: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-H-2Kd antibody (FITC)

Publikationen

Publikationen: York, Rock: "Antigen processing and presentation by the class I major histocompatibility complex." in: Annual review of immunology, Vol. 14, pp. 369-96, 1996 (PubMed).

Noun, Reboul, Abastado et al.: "Alloreactive monoclonal antibodies select Kd molecules with different peptide profiles." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 6, pp. 2455-61, 1996 (PubMed).

Bashuda, Seino, Ra et al.: "Lack of cognate help by CD4+ T cells and anergy of CD8+ T cells are the principal mechanisms for anti-leukocyte function-associated antigen-1/intercellular adhesion molecule-1-induced cardiac allograft tolerance." in: Transplantation, Vol. 63, Issue 1, pp. 113-8, 1997 (PubMed).

Watts: "Capture and processing of exogenous antigens for presentation on MHC molecules." in: Annual review of immunology, Vol. 15, pp. 821-50, 1997 (PubMed).

Pamer, Cresswell: "Mechanisms of MHC class I--restricted antigen processing." in: Annual review of immunology, Vol. 16, pp. 323-58, 1998 (PubMed).

Norian, Allen: "No intrinsic deficiencies in CD8+ T cell-mediated antitumor immunity with aging." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 2, pp. 835-44, 2004 (PubMed).

Ma, Xu, An et al.: "Vaccine with beta-defensin 2-transduced leukemic cells activates innate and adaptive immunity to elicit potent antileukemia responses." in: Cancer research, Vol. 66, Issue 2, pp. 1169-76, 2006 (PubMed).