H-2Kb Antikörper (FITC)

Antigen: H-2Kb

Klonalität: Monoklonal (AF6-88-5)

Wirt: Maus

Reaktivität: Maus

Konjugat: FITC

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN156871

Produktbeschreibung

Immunogen: C57BL mouse splenocytes

Isotyp: IgG2a, IgG2a kappa, kappa chain

Klon: AF6-88-5

Beschreibung: The AF6-88.5 antibody reacts with H-2K MHC class I alloantigen expressed on nucleated cells from mice of the H-2K haplotype. H-2K is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The AF6-88.5 antibody does not cross-react with other haplotypes (d, f, j, k, p, q, r, s, u, v). Distribution: Nucleated cells of the H-2K haplotype Function: Antigen presentation Synonyms: MHC class I Structure: MHC class I

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: immunoprecipitation, and immunohistochemical staining of acetone-fixed frozen sections. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. Product Information

Reinigung: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Lagerung: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-H-2Kb antibody (FITC)

Publikationen

Publikationen: Wall, Lorber, Loken et al.: "Inhibition of proliferation of MIs- and Ia-reactive cloned T cells by a monoclonal antibody against a determinant shared by I-A and I-E." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 131, Issue 3, pp. 1056-64, 1983 (PubMed).

Loken, Stall: "Flow cytometry as an analytical and preparative tool in immunology." in: Journal of immunological methods, Vol. 50, Issue 3, pp. R85-112, 1982 (PubMed).

York, Rock: "Antigen processing and presentation by the class I major histocompatibility complex." in: Annual review of immunology, Vol. 14, pp. 369-96, 1996 (PubMed).

Watts: "Capture and processing of exogenous antigens for presentation on MHC molecules." in: Annual review of immunology, Vol. 15, pp. 821-50, 1997 (PubMed).

Pamer, Cresswell: "Mechanisms of MHC class I--restricted antigen processing." in: Annual review of immunology, Vol. 16, pp. 323-58, 1998 (PubMed).

Andersson, Freland, Johansson et al.: "MHC class I mosaic mice reveal insights into control of Ly49C inhibitory receptor expression in NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 12, pp. 6475-9, 1999 (PubMed).

Shao, Peng, Liao et al.: "A shared epitope of the interphotoreceptor retinoid-binding protein recognized by the CD4+ and CD8+ autoreactive T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 3, pp. 1851-7, 2005 (PubMed).