Signaling Lymphocytic Activation Molecule Family Member 1 (SLAMF1) Antikörper

Antigen: Signaling Lymphocytic Activation Molecule Family Member 1 (SLAMF1)

Synonyme: SLAM, CD150, CDw150, SLAMF1, RGD1560634

Klonalität: Monoklonal (TC15-12F12-2)

Wirt: Ratte

Reaktivität: Maus

Applikation: Durchflusszytometrie (FACS), Immunpräzipitation (IP), Kostimulation, Blockierende Antikörper (Inhibition)

Produktnummer: ABIN156788

Produktbeschreibung

Weitere Bezeichnung: CD150 (SLAM)

Immunogen: Mouse SLAM-human IgG1 fusion protein

Format: Low endotoxin (sterile), Low-endotoxin, azide-free (LEAF)

Isotyp: IgG2a, IgG2a kappa, lambda chain  (Passende Sekundärantikörper)

Klon: TC15-12F12-2

Beschreibung: CD150 is a 75-95 kD member of the immunoglobulin superfamily also known as SLAM (signaling lymphocyte activation molecule) or IPO-3. CD150, a single chain type I transmembrane molecule, is expressed on thymocytes, T cell subsets, B cells, dendritic cells, and endothelial cells. The expression is upregulated upon activation. CD150 expression has been shown to be maintained on Th1, but not Th2 clones. T regulatory cells express a relatively high level of CD150. Antibodies against CD150 have been shown to augment IFN-gamma production by Th1 cells, especially when co-stimulated through the TCR. CD150 associates with the src homology 2-domain-containing protein tyrosine phosphatase SHP-2, and this association is thought to be involved in signal transduction. The TC15-12F12.2 antibody has been reported to enhance the production of IFN-gamma by Th1 cells stimulated through TCR. Distribution: Thymocytes, T cell subset, B lymphocytes, dendritic cells, endothelial cells Function: B cell and dendritic cell costimulation Synonyms: SLAM, IPO-3, Signaling Lymphocyte Activation Molecule Structure: Ig superfamily, 75 - 95 kD

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: immunoprecipitaion, enhancing IFN-gamma production by Th1 cells when stimulated with CD3, and inhibiting CD3 induced T cell proliferation. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN156788). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Reinheit: LEAF

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.

Lagerung: The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.

Forschungsgebiet: CD Antigene

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

C57BL/6 splenocytes stained with TC15-12F12 PE

Publikationen

Publikationen: Cocks, Chang, Carballido et al.: "A novel receptor involved in T-cell activation." in: Nature, Vol. 376, Issue 6537, pp. 260-3, 1995 (PubMed).

Sidorenko, Clark: "Characterization of a cell surface glycoprotein IPO-3, expressed on activated human B and T lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 9, pp. 4614-24, 1993 (PubMed).

Punnonen, Cocks, Carballido et al.: "Soluble and membrane-bound forms of signaling lymphocytic activation molecule (SLAM) induce proliferation and Ig synthesis by activated human B lymphocytes." in: The Journal of experimental medicine, Vol. 185, Issue 6, pp. 993-1004, 1997 (PubMed).

Carlyle, Martin, Mehra et al.: in: (PubMed).

Terrazas, Montero, Terrazas et al.: "Role of the programmed Death-1 pathway in the suppressive activity of alternatively activated macrophages in experimental cysticercosis." in: International journal for parasitology, Vol. 35, Issue 13, pp. 1349-58, 2005 (PubMed).

Forsberg, Prohaska, Katzman et al.: "Differential expression of novel potential regulators in hematopoietic stem cells." in: PLoS genetics, Vol. 1, Issue 3, pp. e28, 2006 (PubMed).

Cannons, Yu, Jankovic et al.: "SAP regulates T cell-mediated help for humoral immunity by a mechanism distinct from cytokine regulation." in: The Journal of experimental medicine, Vol. 203, Issue 6, pp. 1551-65, 2006 (PubMed).

Umemoto, Yamato, Shiratsuchi et al.: "Expression of Integrin beta3 is correlated to the properties of quiescent hemopoietic stem cells possessing the side population phenotype." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 11, pp. 7733-9, 2006 (PubMed).

Sugiyama, Rodriguez, McLean et al.: "Conserved markers of fetal pancreatic epithelium permit prospective isolation of islet progenitor cells by FACS." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 1, pp. 175-80, 2007 (PubMed).

Pimanda, Donaldson, de Bruijn et al.: "The SCL transcriptional network and BMP signaling pathway interact to regulate RUNX1 activity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 3, pp. 840-5, 2007 (PubMed).

Jordan, Fletcher, Pellicci et al.: "Slamf1, the NKT cell control gene Nkt1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 3, pp. 1618-27, 2007 (PubMed).

Jung, Wang, Song et al.: "Annexin II expressed by osteoblasts and endothelial cells regulates stem cell adhesion, homing, and engraftment following transplantation." in: Blood, Vol. 110, Issue 1, pp. 82-90, 2007 (PubMed).