CD19 Molecule (CD19) Antikörper

Antigen: CD19 Molecule (CD19)

Synonyme: B4, MGC12802, AW495831, SPNS1, MGC109570, CD19

Klonalität: Monoklonal (6D5)

Wirt: Ratte

Reaktivität: Maus

Applikation: Durchflusszytometrie (FACS), Western Blot (WB), Immunpräzipitation (IP), Kostimulation

Produktnummer: ABIN156742

Produktbeschreibung

Weitere Bezeichnung: CD19

Format: Low endotoxin (sterile), Low-endotoxin, azide-free (LEAF)

Isotyp: IgG2a, kappa chain  (Passende Sekundärantikörper)

Klon: 6D5

Beschreibung: CD19 is a 95 kD glycoprotein, also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81 forms a molecular complex integral to B cell activation. Distribution: Pro-B to mature B (during development), follicular dendritic cells Function: Modulates B cell activation and differentiation Synonyms: B4 Structure: Ig superfamily, associates with CD21 and CD81, 95 kD

Anwendungen

Applikationshinweise: Additional reported applications include: Western blotting, immunoprecipitation, and costimulation (synergistic with anti-IgM) of B cell activation. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN156742). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Reinheit: LEAF

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.

Lagerung: The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.

Forschungsgebiet: CD Antigene

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

CD57BL/6 mouse splenocytes stained with LEAF(TM) purified 6D5, followed by anti-rat IgG FITC

Publikationen

Publikationen: Tedder, Zhou, Engel: "The CD19/CD21 signal transduction complex of B lymphocytes." in: Immunology today, Vol. 15, Issue 9, pp. 437-42, 1994 (PubMed).

Fearon: "The CD19-CR2-TAPA-1 complex, CD45 and signaling by the antigen receptor of B lymphocytes." in: Current opinion in immunology, Vol. 5, Issue 3, pp. 341-8, 1993 (PubMed).

Krop, de Fougerolles, Hardy et al.: "Self-renewal of B-1 lymphocytes is dependent on CD19." in: European journal of immunology, Vol. 26, Issue 1, pp. 238-42, 1996 (PubMed).

Krop, Shaffer, Fearon et al.: "The signaling activity of murine CD19 is regulated during cell development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 1, pp. 48-56, 1996 (PubMed).

Shoham, Rajapaksa, Boucheix et al.: "The tetraspanin CD81 regulates the expression of CD19 during B cell development in a postendoplasmic reticulum compartment." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 8, pp. 4062-72, 2003 (PubMed).

Goodyear, Narita, Silverman: "In vivo VL-targeted activation-induced apoptotic supraclonal deletion by a microbial B cell toxin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 5, pp. 2870-7, 2004 (PubMed).

Andoniou, van Dommelen, Voigt et al.: "Interaction between conventional dendritic cells and natural killer cells is integral to the activation of effective antiviral immunity." in: Nature immunology, Vol. 6, Issue 10, pp. 1011-9, 2005 (PubMed).

Kamimura, Sawa, Sato et al.: "IL-2 in vivo activities and antitumor efficacy enhanced by an anti-IL-2 mAb." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 1, pp. 306-14, 2006 (PubMed).

Bourdeau, Dube, Heinonen et al.: "TC-PTP-deficient bone marrow stromal cells fail to support normal B lymphopoiesis due to abnormal secretion of interferon-{gamma}." in: Blood, Vol. 109, Issue 10, pp. 4220-8, 2007 (PubMed).

Lawson, Manenkova, Ahamed et al.: "Inhibition of transmethylation down-regulates CD4 T cell activation and curtails development of autoimmunity in a model system." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 8, pp. 5366-74, 2007 (PubMed).

Phan, Grigorova, Okada et al.: "Subcapsular encounter and complement-dependent transport of immune complexes by lymph node B cells." in: Nature immunology, Vol. 8, Issue 9, pp. 992-1000, 2007 (PubMed).