I-Ab Antikörper (FITC)

Antigen: I-Ab

Klonalität: Monoklonal (KH74)

Wirt: Maus

Reaktivität: Maus

Konjugat: FITC

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN156723

Produktbeschreibung

Immunogen: C57BL/10 mouse splenocytes

Isotyp: IgG2a, IgG2a kappa, kappa chain

Klon: KH74

Beschreibung: The KH74 antibody reacts with the I-A MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2 bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The KH74 antibody cross-reacts with the I-A, I-A, I-A, and I-A haplotypes. Distribution: B and activated T cells, APCs of H-2 mice Function: Antigen presentation Synonyms: MHC class II Structure: MHC class II

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity, and immunohistochemical staining of acetone-fixed frozen tissue sections. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Reinigung: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Lagerung: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

C57BL/6 splenocytes stained with KH74 FITC

Publikationen

Publikationen: Hasenkrug, Cory, Stimpfling: "Monoclonal antibodies defining mouse tissue antigens encoded by the H-2 region." in: Immunogenetics, Vol. 25, Issue 2, pp. 136-9, 1987 (PubMed).

Watts: "Capture and processing of exogenous antigens for presentation on MHC molecules." in: Annual review of immunology, Vol. 15, pp. 821-50, 1997 (PubMed).

Pamer, Cresswell: "Mechanisms of MHC class I--restricted antigen processing." in: Annual review of immunology, Vol. 16, pp. 323-58, 1998 (PubMed).

Pappo, Torrey, Castriotta et al.: "Helicobacter pylori infection in immunized mice lacking major histocompatibility complex class I and class II functions." in: Infection and immunity, Vol. 67, Issue 1, pp. 337-41, 1999 (PubMed).