I-Aq Antikörper (Alexa Fluor 647)

Antigen: I-Aq

Klonalität: Monoklonal (KH116)

Wirt: Maus

Reaktivität: Maus

Konjugat: Alexa Fluor 647

Applikation: Durchflusszytometrie (FACS), Immunhistochemie (Gefrierschnitte) (IHC (fro)), Immunfluoreszenz (IF)

Produktnummer: ABIN156717

Produktbeschreibung

Immunogen: B10.Q mouse splenocytes

Isotyp: IgG2b, IgG2b kappa, kappa chain

Klon: KH116

Beschreibung: The KH116 antibody reacts with the I-A MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2 bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The KH116 antibody cross-reacts with I-A and I-A, reactivity with other haplotypes has not been reported. Distribution: B and activated T cells, APCs of H-2 mice Function: Antigen presentation Synonyms: MHC class II Structure: MHC class II

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity, immunoprecipitation, and immunohistochemical staining of acetone-fixed frozen sections. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.

Reinigung: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Lagerung: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-I-Aq antibody (Alexa Fluor 647)

Publikationen

Publikationen: Watts: "Capture and processing of exogenous antigens for presentation on MHC molecules." in: Annual review of immunology, Vol. 15, pp. 821-50, 1997 (PubMed).

Pamer, Cresswell: "Mechanisms of MHC class I--restricted antigen processing." in: Annual review of immunology, Vol. 16, pp. 323-58, 1998 (PubMed).

Nakagawa, Brissette, Lira et al.: "Impaired invariant chain degradation and antigen presentation and diminished collagen-induced arthritis in cathepsin S null mice." in: Immunity, Vol. 10, Issue 2, pp. 207-17, 1999 (PubMed).

Zuo, Cullen, DeLay et al.: "A single-chain class II MHC-IgG3 fusion protein inhibits autoimmune arthritis by induction of antigen-specific hyporesponsiveness." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 5, pp. 2554-9, 2002 (PubMed).