H-2Kb/H-2Dd Antikörper (Alexa Fluor 647)

Antigen: H-2Kb/H-2Dd

Klonalität: Monoklonal (34-1-2S)

Wirt: Maus

Reaktivität: Maus

Konjugat: Alexa Fluor 647

Applikation: Durchflusszytometrie (FACS), Immunhistochemie (Gefrierschnitte) (IHC (fro)), Immunfluoreszenz (IF)

Produktnummer: ABIN156682

Produktbeschreibung

Immunogen: BDF Splenocytes

Isotyp: IgG2a, IgG2a kappa, kappa chain

Klon: 34-1-2S

Beschreibung: The 34-1-2S antibody reacts with the H-2K/H-2D MHC class I alloantigens expressed on nucleated cells from mice of the H-2K/H-2D haplotype. H-2K/H-2D is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 34-1-2S antibody cross-reacts with H-2K MHC class I alloantigens of the b, s, r, q, or p haplotypes. Distribution: Nucleated cells of the H-2K/H-2D haplotype Function: Antigen presentation Synonyms: MHC class I Structure: MHC class I

Spezifität: Cross-reacts with h-2 mhc class i alloantigens of the b

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: immunoprecipitation, complement-mediated cytotoxicity, and immunohistochemistry of acetone-fixed frozen sections. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.

Reinigung: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Lagerung: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-H-2Kb/H-2Dd antibody (Alexa Fluor 647)

Publikationen

Publikationen: Ozato, Mayer, Sachs: "Monoclonal antibodies to mouse major histocompatibility complex antigens." in: Transplantation, Vol. 34, Issue 3, pp. 113-20, 1982 (PubMed).

York, Rock: "Antigen processing and presentation by the class I major histocompatibility complex." in: Annual review of immunology, Vol. 14, pp. 369-96, 1996 (PubMed).

Watts: "Capture and processing of exogenous antigens for presentation on MHC molecules." in: Annual review of immunology, Vol. 15, pp. 821-50, 1997 (PubMed).

Pamer, Cresswell: "Mechanisms of MHC class I--restricted antigen processing." in: Annual review of immunology, Vol. 16, pp. 323-58, 1998 (PubMed).