H-2Kb/H-2Dd Antikörper (Biotin)

Antigen: H-2Kb/H-2Dd

Klonalität: Monoklonal (34-1-2S)

Wirt: Maus

Reaktivität: Maus

Konjugat: Biotin

Applikation: Durchflusszytometrie (FACS), Immunhistochemie (IHC)

Produktnummer: ABIN156673

Produktbeschreibung

Immunogen: BDF Splenocytes

Isotyp: IgG2a, IgG2a kappa, kappa chain

Klon: 34-1-2S

Beschreibung: The 34-1-2S antibody reacts with the H-2K/H-2D MHC class I alloantigens expressed on nucleated cells from mice of the H-2K/H-2D haplotype. H-2K/H-2D is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 34-1-2S antibody cross-reacts with H-2K MHC class I alloantigens of the b, s, r, q, or p haplotypes. Distribution: Nucleated cells of the H-2K/H-2D haplotype Function: Antigen presentation Synonyms: MHC class I Structure: MHC class I

Spezifität: Cross-reacts with h-2 mhc class i alloantigens of the b

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: immunoprecipitation, complement-mediated cytotoxicity, and immunohistochemistry of acetone-fixed frozen sections. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.25 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Reinigung: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Lagerung: The antibody solution should be stored undiluted at 4°C. Do not freeze.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

C57BL/6 mouse splenocytes stained with 34-1-2S PE C3H/He mouse splenocytes stained with 34-1-2S PE

C3H/He mouse splenocytes stained with 34-1-2S PE

Publikationen

Publikationen: Ozato, Mayer, Sachs: "Monoclonal antibodies to mouse major histocompatibility complex antigens." in: Transplantation, Vol. 34, Issue 3, pp. 113-20, 1982 (PubMed).

York, Rock: "Antigen processing and presentation by the class I major histocompatibility complex." in: Annual review of immunology, Vol. 14, pp. 369-96, 1996 (PubMed).

Watts: "Capture and processing of exogenous antigens for presentation on MHC molecules." in: Annual review of immunology, Vol. 15, pp. 821-50, 1997 (PubMed).

Pamer, Cresswell: "Mechanisms of MHC class I--restricted antigen processing." in: Annual review of immunology, Vol. 16, pp. 323-58, 1998 (PubMed).