I-Ab (Abetab) Antikörper (Alexa Fluor 647)

Antigen: I-Ab (Abetab)

Klonalität: Monoklonal (25-09-2017)

Wirt: Maus

Reaktivität: Maus

Konjugat: Alexa Fluor 647

Applikation: Durchflusszytometrie (FACS), Immunhistochemie (Gefrierschnitte) (IHC (fro)), Immunfluoreszenz (IF)

Produktnummer: ABIN156643

Produktbeschreibung

Immunogen: C3H.SW splenocytes

Isotyp: IgG2a, IgG2a kappa, kappa chain

Klon: 25-09-2017

Beschreibung: The 25-9-17 antibody reacts with the beta chain of the I-A MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2 bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The 25-9-17 antibody cross-reacts with alloantigens of the H-2 haplotypes. Distribution: B and activated T cells, APCs of H-2 mice Function: Antigen presentation Synonyms: MHC class II Structure: ß chain of I-A MHC class II

Spezifität: Cross-reacts with alloantigens of h-2 haplotypes

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity, immunoprecipitation, and immunohistochemical staining of acetone-fixed frozen sections. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN156644). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.

Reinigung: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide

Lagerung: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-I-Ab (Abetab) antibody (Alexa Fluor 647)

Publikationen

Publikationen: Cohn, Glimcher, Waldmann et al.: "Identification of functional regions on the I-Ab molecule by site-directed mutagenesis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 3, pp. 747-51, 1986 (PubMed).

Wall, Lorber, Loken et al.: "Inhibition of proliferation of MIs- and Ia-reactive cloned T cells by a monoclonal antibody against a determinant shared by I-A and I-E." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 131, Issue 3, pp. 1056-64, 1983 (PubMed).

Ozato, Sachs: "Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 126, Issue 1, pp. 317-21, 1981 (PubMed).

Watts: "Capture and processing of exogenous antigens for presentation on MHC molecules." in: Annual review of immunology, Vol. 15, pp. 821-50, 1997 (PubMed).

Pamer, Cresswell: "Mechanisms of MHC class I--restricted antigen processing." in: Annual review of immunology, Vol. 16, pp. 323-58, 1998 (PubMed).

Nikolic, Gardner, Scadden et al.: "Normal development in porcine thymus grafts and specific tolerance of human T cells to porcine donor MHC." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 6, pp. 3402-7, 1999 (PubMed).

Khanna, Morelli, Zhong et al.: "Effects of liver-derived dendritic cell progenitors on Th1- and Th2-like cytokine responses in vitro and in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 3, pp. 1346-54, 2000 (PubMed).

Meuter, Schaerli, Roos et al.: "Murine CXCL14 is dispensable for dendritic cell function and localization within peripheral tissues." in: Molecular and cellular biology, Vol. 27, Issue 3, pp. 983-92, 2007 (PubMed).