I-Ab (Abetab) Antikörper (Biotin)

Antigen: I-Ab (Abetab)

Klonalität: Monoklonal (25-09-2017)

Wirt: Maus

Reaktivität: Maus

Konjugat: Biotin

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN156636

Produktbeschreibung

Immunogen: C3H.SW splenocytes

Isotyp: IgG2a, IgG2a kappa, kappa chain

Klon: 25-09-2017

Beschreibung: The 25-9-17 antibody reacts with the beta chain of the I-A MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2 bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The 25-9-17 antibody cross-reacts with alloantigens of the H-2 haplotypes. Distribution: B and activated T cells, APCs of H-2 mice Function: Antigen presentation Synonyms: MHC class II Structure: beta chain of I-A MHC class II

Spezifität: Cross-reacts with alloantigens of h-2 haplotypes

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity, immunoprecipitation, and immunohistochemical staining of acetone-fixed frozen sections. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN156644). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.25 µg per 10cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Reinigung: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Lagerung: The antibody solution should be stored undiluted at 4°C. Do not freeze.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

BALB/c mouse splenocytes stained with 25-9-17 FITC

Publikationen

Publikationen: Cohn, Glimcher, Waldmann et al.: "Identification of functional regions on the I-Ab molecule by site-directed mutagenesis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 3, pp. 747-51, 1986 (PubMed).

Wall, Lorber, Loken et al.: "Inhibition of proliferation of MIs- and Ia-reactive cloned T cells by a monoclonal antibody against a determinant shared by I-A and I-E." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 131, Issue 3, pp. 1056-64, 1983 (PubMed).

Ozato, Sachs: "Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 126, Issue 1, pp. 317-21, 1981 (PubMed).

Watts: "Capture and processing of exogenous antigens for presentation on MHC molecules." in: Annual review of immunology, Vol. 15, pp. 821-50, 1997 (PubMed).

Pamer, Cresswell: "Mechanisms of MHC class I--restricted antigen processing." in: Annual review of immunology, Vol. 16, pp. 323-58, 1998 (PubMed).

Nikolic, Gardner, Scadden et al.: "Normal development in porcine thymus grafts and specific tolerance of human T cells to porcine donor MHC." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 6, pp. 3402-7, 1999 (PubMed).

Khanna, Morelli, Zhong et al.: "Effects of liver-derived dendritic cell progenitors on Th1- and Th2-like cytokine responses in vitro and in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 3, pp. 1346-54, 2000 (PubMed).

Meuter, Schaerli, Roos et al.: "Murine CXCL14 is dispensable for dendritic cell function and localization within peripheral tissues." in: Molecular and cellular biology, Vol. 27, Issue 3, pp. 983-92, 2007 (PubMed).