H-2Dk Antikörper

Antigen: H-2Dk

Klonalität: Monoklonal (15-05-2005)

Wirt: Maus

Reaktivität: Maus

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN156517

Produktbeschreibung

Immunogen: C3H mouse splenocytes

Format: Purified

Isotyp: IgG2a, IgG2a kappa, kappa chain  (Passende Sekundärantikörper)

Klon: 15-05-2005

Beschreibung: The 15-5-5 antibody reacts with the H-2D MHC class I alloantigen expressed on nucleated cells from mice of the H-2D haplotype. H-2D is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 15-5-5 antibody weakly cross-reacts with H-2K and with cells from mice of the H-2 haplotype. Reactivity with other haplotypes (e.g., a,b,p,q,r,s) has not been reported. Distribution: Nucleated cells of the H-2D haplotype Function: Antigen presentation Synonyms: MHC class I Structure: MHC class I

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 1.0 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Lagerung: The antibody solution should be stored undiluted at 4°C.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

C3H/He mouse splenocytes stained with 15-5-5 FITC

Publikationen

Publikationen: Ozato, Mayer, Sachs: "Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 124, Issue 2, pp. 533-40, 1980 (PubMed).

York, Rock: "Antigen processing and presentation by the class I major histocompatibility complex." in: Annual review of immunology, Vol. 14, pp. 369-96, 1996 (PubMed).

Watts: "Capture and processing of exogenous antigens for presentation on MHC molecules." in: Annual review of immunology, Vol. 15, pp. 821-50, 1997 (PubMed).

Yamamoto, Fujihashi, Kawabata et al.: "A mucosal intranet: intestinal epithelial cells down-regulate intraepithelial, but not peripheral, T lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 5, pp. 2188-96, 1998 (PubMed).

Pamer, Cresswell: "Mechanisms of MHC class I--restricted antigen processing." in: Annual review of immunology, Vol. 16, pp. 323-58, 1998 (PubMed).