I-Ak (Aalphak) Antikörper (Alexa Fluor 488)

Antigen: I-Ak (Aalphak)

Klonalität: Monoklonal (11-05-2002)

Wirt: Maus

Reaktivität: Maus

Konjugat: Alexa Fluor 488

Applikation: Durchflusszytometrie (FACS), Immunhistochemie (Gefrierschnitte) (IHC (fro)), Immunfluoreszenz (IF)

Produktnummer: ABIN156500

Produktbeschreibung

Immunogen: CKB mouse splenocytes

Isotyp: IgG2b, IgG2b kappa, kappa chain

Klon: 11-05-2002

Beschreibung: The 11-5.2 antibody reacts with the alpha chain of the I-A MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2 bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The 11-5.2 antibody cross-reacts with cells from mice bearing the H-2 haplotype. Reactivity with other H-2 haplotypes (e.g., b, d, f, p, q, s) has not been observed. The 11-5.2 antibody has been reported to block antigen presentation and induce B cell differentiation. Distribution: B and activated T cells, APCs of H-2 mice Function: Antigen presentation Synonyms: MHC class II Structure: a chain of MHC class II

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: immunoprecipitation, blocking of MHC class II - restricted CD4 T cell responses, induction of B cell differentiation, and immunohistochemical staining of acetone-fixed frozen sections. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.

Reinigung: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Lagerung: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-I-Ak (Aalphak) antibody (Alexa Fluor 488)

Publikationen

Publikationen: Oi, Jones, Goding et al.: "Properties of monoclonal antibodies to mouse Ig allotypes, H-2, and Ia antigens." in: Current topics in microbiology and immunology, Vol. 81, pp. 115-20, 1978 (PubMed).

Formby, Miller: "Autologous CD4 T-cell responses to ectopic class II major histocompatibility complex antigen-expressing single-cell islet cells: an in vitro insight into the pathogenesis of lymphocytic insulitis in nonobese diabetic mice." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 7, pp. 2438-42, 1990 (PubMed).

Bishop, Frelinger: "Haplotype-specific differences in signaling by transfected class II molecules to a Ly-1+ B-cell clone." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 15, pp. 5933-7, 1989 (PubMed).

Kappler, Skidmore, White et al.: "Antigen-inducible, H-2-restricted, interleukin-2-producing T cell hybridomas. Lack of independent antigen and H-2 recognition." in: The Journal of experimental medicine, Vol. 153, Issue 5, pp. 1198-214, 1981 (PubMed).

Ardehali, Laks, Drinkwater et al.: "Vascular cell adhesion molecule-1 is induced on vascular endothelia and medial smooth muscle cells in experimental cardiac allograft vasculopathy." in: Circulation, Vol. 92, Issue 3, pp. 450-6, 1995 (PubMed).

Watts: "Capture and processing of exogenous antigens for presentation on MHC molecules." in: Annual review of immunology, Vol. 15, pp. 821-50, 1997 (PubMed).

Pamer, Cresswell: "Mechanisms of MHC class I--restricted antigen processing." in: Annual review of immunology, Vol. 16, pp. 323-58, 1998 (PubMed).

Turnquist, Raimondi, Zahorchak et al.: "Rapamycin-conditioned dendritic cells are poor stimulators of allogeneic CD4+ T cells, but enrich for antigen-specific Foxp3+ T regulatory cells and promote organ transplant tolerance." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 11, pp. 7018-31, 2007 (PubMed).