I-Ak (Aalphak) Antikörper

Antigen: I-Ak (Aalphak)

Klonalität: Monoklonal (11-05-2002)

Wirt: Maus

Reaktivität: Maus

Applikation: Durchflusszytometrie (FACS), Immunpräzipitation (IP), Immunhistochemie (Gefrierschnitte) (IHC (fro))

Produktnummer: ABIN156493

Produktbeschreibung

Immunogen: CKB mouse splenocytes

Format: Purified

Isotyp: IgG2b, IgG2b kappa, kappa chain  (Passende Sekundärantikörper)

Klon: 11-05-2002

Beschreibung: The 11-5.2 antibody reacts with the alpha chain of the I-A MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2 bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The 11-5.2 antibody cross-reacts with cells from mice bearing the H-2 haplotype. Reactivity with other H-2 haplotypes (e.g., b, d, f, p, q, s) has not been observed. The 11-5.2 antibody has been reported to block antigen presentation and induce B cell differentiation. Distribution: B and activated T cells, APCs of H-2 mice Function: Antigen presentation Synonyms: MHC class II Structure: alpha chain of MHC class II

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: immunoprecipitation, blocking of MHC class II - restricted CD4 T cell responses, induction of B cell differentiation, and immunohistochemical staining of acetone-fixed frozen sections. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Lagerung: The antibody solution should be stored undiluted at 4 °C.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

BALB/c mouse splenocytes stained with 11-5.2 biotin and detected with Sav-PE C3H/He mouse splenocytes stained with 11-5.2 biotin and detected with Sav-PE

C3H/He mouse splenocytes stained with 11-5.2 biotin and detected with Sav-PE

Publikationen

Publikationen: Oi, Jones, Goding et al.: "Properties of monoclonal antibodies to mouse Ig allotypes, H-2, and Ia antigens." in: Current topics in microbiology and immunology, Vol. 81, pp. 115-20, 1978 (PubMed).

Formby, Miller: "Autologous CD4 T-cell responses to ectopic class II major histocompatibility complex antigen-expressing single-cell islet cells: an in vitro insight into the pathogenesis of lymphocytic insulitis in nonobese diabetic mice." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 7, pp. 2438-42, 1990 (PubMed).

Bishop, Frelinger: "Haplotype-specific differences in signaling by transfected class II molecules to a Ly-1+ B-cell clone." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 15, pp. 5933-7, 1989 (PubMed).

Kappler, Skidmore, White et al.: "Antigen-inducible, H-2-restricted, interleukin-2-producing T cell hybridomas. Lack of independent antigen and H-2 recognition." in: The Journal of experimental medicine, Vol. 153, Issue 5, pp. 1198-214, 1981 (PubMed).

Ardehali, Laks, Drinkwater et al.: "Vascular cell adhesion molecule-1 is induced on vascular endothelia and medial smooth muscle cells in experimental cardiac allograft vasculopathy." in: Circulation, Vol. 92, Issue 3, pp. 450-6, 1995 (PubMed).

Watts: "Capture and processing of exogenous antigens for presentation on MHC molecules." in: Annual review of immunology, Vol. 15, pp. 821-50, 1997 (PubMed).

Pamer, Cresswell: "Mechanisms of MHC class I--restricted antigen processing." in: Annual review of immunology, Vol. 16, pp. 323-58, 1998 (PubMed).

Turnquist, Raimondi, Zahorchak et al.: "Rapamycin-conditioned dendritic cells are poor stimulators of allogeneic CD4+ T cells, but enrich for antigen-specific Foxp3+ T regulatory cells and promote organ transplant tolerance." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 11, pp. 7018-31, 2007 (PubMed).