I-Ak (Abetak) Antikörper (FITC)

Antigen: I-Ak (Abetak)

Klonalität: Monoklonal (10-03-2006)

Wirt: Maus

Reaktivität: Maus

Konjugat: FITC

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN156485

Produktbeschreibung

Immunogen: C3H mouse splenocytes

Isotyp: IgG2a, IgG2a kappa, kappa chain

Klon: 10-03-2006

Beschreibung: The 10-3.6 antibody reacts with the beta chain of the I-A MHC class II alloantigen. This class II molecule is expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2 bearing mice and involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The 10-3.6 antibody cross-reacts with I-A antigens, does not react with other haplotypes (e.g., b, d, p, q). Distribution: B and activated T cells, APCs of H-2 mice Function: Antigen presentation Synonyms: MHC class II Structure: beta chain of MHC class II

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include immunoprecipitation, protection against autoimmune IDDM, in vitro blocking of antigen-specific MHC-restricted responses and in vivo inhibition of lymphoma growth, and immunohistochemical staining of acetone-fixed frozen sections. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Reinigung: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Lagerung: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

C3H/He mouse splenocytes stained with 10-3.6 PE BALB/c mouse splenocytes stained with 10-3.6 biotin and detected with Sav-PE

BALB/c mouse splenocytes stained with 10-3.6 biotin and detected with Sav-PE

Publikationen

Publikationen: Alisauskas, Ponzio: "In vivo growth inhibition of Ia+ lymphomas in SJL mice treated with I-As-reactive monoclonal antibody." in: Immunopharmacology, Vol. 12, Issue 1, pp. 1-9, 1986 (PubMed).

Kappler, Skidmore, White et al.: "Antigen-inducible, H-2-restricted, interleukin-2-producing T cell hybridomas. Lack of independent antigen and H-2 recognition." in: The Journal of experimental medicine, Vol. 153, Issue 5, pp. 1198-214, 1981 (PubMed).

Kupinski, Plunkett, Freed: "Assignment of antigenic determinants to separated I-A kappa chains." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 130, Issue 5, pp. 2277-81, 1983 (PubMed).

Watts: "Capture and processing of exogenous antigens for presentation on MHC molecules." in: Annual review of immunology, Vol. 15, pp. 821-50, 1997 (PubMed).

Pamer, Cresswell: "Mechanisms of MHC class I--restricted antigen processing." in: Annual review of immunology, Vol. 16, pp. 323-58, 1998 (PubMed).