NK-1.1 Antikörper

Antigen: NK-1.1

Klonalität: Monoklonal (PK136)

Wirt: Maus

Reaktivität: Maus

Applikation: Durchflusszytometrie (FACS), Immunpräzipitation (IP), Abreicherung (Depletion), Blockierende Antikörper (Inhibition), Immunhistochemie (Gefrierschnitte) (IHC (fro)), Immunhistochemie (IHC), Immunfluoreszenz (IF)

Produktnummer: ABIN156387

Produktbeschreibung

Immunogen: NK-1 cells from mouse spleen and bone marrow

Format: Low endotoxin (sterile), Low-endotoxin, azide-free (LEAF)

Isotyp: IgG2a, kappa chain  (Passende Sekundärantikörper)

Klon: PK136

Beschreibung: NK-1.1 surface antigen is encoded by the NKR-P1B/NKR-P1C gene, also known as CD161b/CD161c and Ly-55. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN-gamma production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers. The PK136 antibody has been reported to deplete NK cells, induce redirected lysis, block NK cell function, and induce NK cell proliferation. Distribution: NK and NK-T cells in the NK1.1 mouse strains (C57BL, FVB/N, NZB) Function: NK cell activation, IFN-gamma production, cytotoxic granule release Synonyms: NKR-P1C, NKR-P1B, Ly-55, CD161b, CD161c Structure: NKR-P1 gene family

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: immunoprecipitation, complement-dependent cytotoxicity, in vivo depletion, mediation of in vitro redirected lysis, blocking of NK cell function, induction of proliferation, and immunohistochemistry of frozen sections and immunofluorescence microscopy. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN156387). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.25 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Reinheit: LEAF

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.

Lagerung: The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

C57BL/6 splenocytes stained with PK136 FITC and NKG2D PE

Publikationen

Publikationen: Giorda, Trucco: "Mouse NKR-P1. A family of genes selectively coexpressed in adherent lymphokine-activated killer cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 5, pp. 1701-8, 1991 (PubMed).

Karlhofer, Yokoyama: "Stimulation of murine natural killer (NK) cells by a monoclonal antibody specific for the NK1.1 antigen. IL-2-activated NK cells possess additional specific stimulation pathways." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 10, pp. 3662-73, 1991 (PubMed).

Sentman, Hackett, Moore et al.: "Pan natural killer cell monoclonal antibodies and their relationship to the NK1.1 antigen." in: Hybridoma, Vol. 8, Issue 6, pp. 605-14, 1990 (PubMed).

Sentman, Kumar, Koo et al.: "Effector cell expression of NK1.1, a murine natural killer cell-specific molecule, and ability of mice to reject bone marrow allografts." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 6, pp. 1847-53, 1989 (PubMed).

Koo, Dumont, Tutt et al.: "The NK-1.1(-) mouse: a model to study differentiation of murine NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 137, Issue 12, pp. 3742-7, 1987 (PubMed).

Koo, Peppard: "Establishment of monoclonal anti-Nk-1.1 antibody." in: Hybridoma, Vol. 3, Issue 3, pp. 301-3, 1985 (PubMed).

Yokoyama, Seaman: "The Ly-49 and NKR-P1 gene families encoding lectin-like receptors on natural killer cells: the NK gene complex." in: Annual review of immunology, Vol. 11, pp. 613-35, 1993 (PubMed).

Drobyski, Majewski: "Treatment of donor mice with an alpha beta T-cell receptor monoclonal antibody induces prolonged T-cell nonresponsiveness and effectively prevents lethal graft-versus-host disease in murine recipients of major histocompatibility complex (MHC)-matched and " in: Blood, Vol. 87, Issue 12, pp. 5355-69, 1996 (PubMed).

Lanier: "Natural killer cells: from no receptors to too many." in: Immunity, Vol. 6, Issue 4, pp. 371-8, 1997 (PubMed).

Reichlin, Yokoyama: "Natural killer cell proliferation induced by anti-NK1.1 and IL-2." in: Immunology and cell biology, Vol. 76, Issue 2, pp. 143-52, 1998 (PubMed).

Kung, Su, Shannon et al.: "The NKR-P1B gene product is an inhibitory receptor on SJL/J NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 10, pp. 5876-87, 1999 (PubMed).

Carlyle, Martin, Mehra et al.: "Mouse NKR-P1B, a novel NK1.1 antigen with inhibitory function." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 10, pp. 5917-23, 1999 (PubMed).

Kanwar, Shen, Kanwar et al.: "Effects of survivin antagonists on growth of established tumors and B7-1 immunogene therapy." in: Journal of the National Cancer Institute, Vol. 93, Issue 20, pp. 1541-52, 2001 (PubMed).

Andoniou, van Dommelen, Voigt et al.: "Interaction between conventional dendritic cells and natural killer cells is integral to the activation of effective antiviral immunity." in: Nature immunology, Vol. 6, Issue 10, pp. 1011-9, 2005 (PubMed).