Mac-3 Antikörper

Antigen: Mac-3

Klonalität: Monoklonal (M3-84)

Wirt: Ratte

Reaktivität: Maus

Applikation: Durchflusszytometrie (FACS), Immunpräzipitation (IP), Immunhistochemie (Gefrierschnitte) (IHC (fro))

Produktnummer: ABIN156365

Produktbeschreibung

Immunogen: Membrane glycoproteins from C57BL/6 mouse peritoneal exudate cells

Format: Purified

Isotyp: IgG1, kappa chain  (Passende Sekundärantikörper)

Klon: M3-84

Beschreibung: Mac-3 is a 110 kD type I membrane glycoprotein, also known as CD107b and LAMP-2. It is expressed on lysosomal membranes and the plasma membrane of macrophages and some myeloid cell lines. In the bone marrow, few cells display Mac-3 antigen on the surface, but a large proportion express Mac-3 in the cytoplasm. CD107b has been identified as a ligand for galaptin, an S-type lectin present in the extracellular matrix. Mac-3/CD107b is upregulated in some tumors and increased expression has been correlated with enhanced metastatic potential. Distribution: Macrophages, some myeloid cell lines Function: Differentiation and activation Synonyms: CD107b, LAMP-2 Structure: 110 kD

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: immunoprecipitation and immunohistochemical staining of acetone-fixed frozen sections. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.25 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Lagerung: The antibody solution should be stored undiluted at 4 °C.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Thioglycolate-elicited peritoneal macrophages stained with M3/84 PE

Publikationen

Publikationen: Chen, Murphy, Willingham et al.: "Identification of two lysosomal membrane glycoproteins." in: The Journal of cell biology, Vol. 101, Issue 1, pp. 85-95, 1985 (PubMed).

Ho, Springer: "Tissue distribution, structural characterization, and biosynthesis of Mac-3, a macrophage surface glycoprotein exhibiting molecular weight heterogeneity." in: The Journal of biological chemistry, Vol. 258, Issue 1, pp. 636-42, 1983 (PubMed).

Flotte, Springer, Thorbecke: "Dendritic cell and macrophage staining by monoclonal antibodies in tissue sections and epidermal sheets." in: The American journal of pathology, Vol. 111, Issue 1, pp. 112-24, 1983 (PubMed).

Ralph, Ho, Litcofsky et al.: "Expression and induction in vitro of macrophage differentiation antigens on murine cell lines." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 130, Issue 1, pp. 108-14, 1983 (PubMed).

Springer: "Monoclonal antibody analysis of complex biological systems. Combination of cell hybridization and immunoadsorbents in a novel cascade procedure and its application to the macrophage cell surface." in: The Journal of biological chemistry, Vol. 256, Issue 8, pp. 3833-9, 1981 (PubMed).

Kano, Bashuda, Yagita et al.: "A crucial role of host CD80 and CD86 in rat cardiac xenograft rejection in mice." in: Transplantation, Vol. 65, Issue 6, pp. 837-43, 1998 (PubMed).