Lymphocyte Antigen 6 Complex, Locus G (Ly6g) Antikörper

Antigen: Lymphocyte Antigen 6 Complex, Locus G (Ly6g)

Synonyme: Gr1, Gr-1, Ly-6G

Klonalität: Monoklonal (RB6-8C5)

Wirt: Ratte

Reaktivität: Maus

Applikation: Durchflusszytometrie (FACS), Immunpräzipitation (IP), Abreicherung (Depletion), Immunhistochemie (Gefrierschnitte) (IHC (fro)), Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB)

Produktnummer: ABIN156353

Produktbeschreibung

Weitere Bezeichnung: Ly-6G (Gr-1)

Format: Low endotoxin (sterile), Low-endotoxin, azide-free (LEAF)

Isotyp: IgG2b, kappa chain

Klon: RB6-8C5

Beschreibung: Gr-1 is a 21-25 kD protein also known as Ly-6G/Ly-6C. This myeloid differentiation antigen is a glycosylphosphatidylinositol (GPI)-linked protein expressed on granulocytes and macrophages. In bone marrow, the expression levels of Gr-1 directly correlate with granulocyte differentiation and maturation, Gr-1 is also transiently expressed on bone marrow cells in the monocyte lineage. Gr-1 cells have recently been implicated in the development of antitumor responses. The RB6-8C5 antibody has been used to identify peripheral blood neutrophils and deplete granulocytes in vivo. Distribution: Granulocytes, monocytes Synonyms: Gr-1 Structure: 21-25 kD

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: in vitro complement-mediated cytotoxicity, in vivo depletion, immunoprecipitation, immunohistochemical staining of zinc-fixed paraffin-embedded sections and acetone-fixed frozen sections, and Western blotting. Special Note: This antibody is not recommended for formalin-fixed paraffin-embedded sections. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN156353). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.25 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Reinheit: LEAF

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.

Lagerung: The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.

Forschungsgebiet: Zell-/Gewebemarker, Immunologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

C57BL/6 bone marrow cells stained with RB6-8C5 FITC and CD11b PE

Publikationen

Publikationen: Jutila, Kroese, Jutila et al.: "Ly-6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon-gamma." in: European journal of immunology, Vol. 18, Issue 11, pp. 1819-26, 1989 (PubMed).

Brummer, Sugar, Stevens: "Immunological activation of polymorphonuclear neutrophils for fungal killing: studies with murine cells and blastomyces dermatitidis in vitro." in: Journal of leukocyte biology, Vol. 36, Issue 4, pp. 505-20, 1984 (PubMed).

Stoppacciaro, Melani, Parenza et al.: "Regression of an established tumor genetically modified to release granulocyte colony-stimulating factor requires granulocyte-T cell cooperation and T cell-produced interferon gamma." in: The Journal of experimental medicine, Vol. 178, Issue 1, pp. 151-61, 1993 (PubMed).

Czuprynski, Brown, Maroushek et al.: "Administration of anti-granulocyte mAb RB6-8C5 impairs the resistance of mice to Listeria monocytogenes infection." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 4, pp. 1836-46, 1994 (PubMed).

Fleming, Fleming, Malek: "Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 5, pp. 2399-408, 1993 (PubMed).

Tumpey, Chen, Oakes et al.: "Neutrophil-mediated suppression of virus replication after herpes simplex virus type 1 infection of the murine cornea." in: Journal of virology, Vol. 70, Issue 2, pp. 898-904, 1996 (PubMed).

Goni, Alcaide, Fresno: "Immunosuppression during acute Trypanosoma cruzi infection: involvement of Ly6G (Gr1(+))CD11b(+ )immature myeloid suppressor cells." in: International immunology, Vol. 14, Issue 10, pp. 1125-34, 2002 (PubMed).

Brown, Blaho, Loiacono: "Treatment of mice with the neutrophil-depleting antibody RB6-8C5 results in early development of experimental lyme arthritis via the recruitment of Gr-1- polymorphonuclear leukocyte-like cells." in: Infection and immunity, Vol. 72, Issue 9, pp. 4956-65, 2004 (PubMed).

Engwerda, Ato, Staeger et al.: "Distinct roles for lymphotoxin-alpha and tumor necrosis factor in the control of Leishmania donovani infection." in: The American journal of pathology, Vol. 165, Issue 6, pp. 2123-33, 2004 (PubMed).

Andoniou, van Dommelen, Voigt et al.: "Interaction between conventional dendritic cells and natural killer cells is integral to the activation of effective antiviral immunity." in: Nature immunology, Vol. 6, Issue 10, pp. 1011-9, 2005 (PubMed).

Dzhagalov, St John, He: "The antiapoptotic protein Mcl-1 is essential for the survival of neutrophils but not macrophages." in: Blood, Vol. 109, Issue 4, pp. 1620-6, 2007 (PubMed).

Heuser, Argiropoulos, Kuchenbauer et al.: "MN1 overexpression induces acute myeloid leukemia in mice and predicts ATRA resistance in patients with AML." in: Blood, Vol. 110, Issue 5, pp. 1639-47, 2007 (PubMed).

Fazilleau, Eisenbraun, Malherbe et al.: "Lymphoid reservoirs of antigen-specific memory T helper cells." in: Nature immunology, Vol. 8, Issue 7, pp. 753-61, 2007 (PubMed).