Ly-51 Antikörper (Alexa Fluor 647)

Antigen: Ly-51

Klonalität: Monoklonal (6C3)

Wirt: Ratte

Reaktivität: Maus

Konjugat: Alexa Fluor 647

Applikation: Durchflusszytometrie (FACS), Immunhistochemie (Gefrierschnitte) (IHC (fro)), Immunfluoreszenz (IF)

Produktnummer: ABIN156339

Produktbeschreibung

Immunogen: A C57L mouse Pre-B lymphoma cell line (L1-2) plus

Isotyp: IgG2a, kappa chain

Klon: 6C3

Beschreibung: Ly-51 is a 140 kD protein also known as 6C3/BP-1. It is a homodimeric cell-surface glycoprotein with aminopeptidase A (APA) activity. Ly-51 is expressed on B cell progenitors, bone marrow stromal cell lines, thymic dendritic cells, and cortical epithelial cells. Ly-51 expression can be upregulated by IL-7 stimulation. Distribution: B progenitors, bone marrow stromal lines, thymic dendritic cells, cortical epithelial cells Function: Aminopeptidase, may function in IL-7-driven proliferation of B cell precursors Synonyms: 6C3/BP-1 Structure: Aminopeptidase A, 140 kD

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: immunoprecipitation and immunohistochemical staining of acetone-fixed frozen sections. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.

Reinigung: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Lagerung: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Forschungsgebiet: Zell-/Gewebemarker, Immunologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-Ly-51 antibody (Alexa Fluor 647)

Publikationen

Publikationen: Surh, Ernst, Sprent: "Growth of epithelial cells in the thymic medulla is under the control of mature T cells." in: The Journal of experimental medicine, Vol. 176, Issue 2, pp. 611-6, 1992 (PubMed).

Hardy, Carmack, Shinton et al.: "Resolution and characterization of pro-B and pre-pro-B cell stages in normal mouse bone marrow." in: The Journal of experimental medicine, Vol. 173, Issue 5, pp. 1213-25, 1991 (PubMed).

Sherwood, Weissman: "The growth factor IL-7 induces expression of a transformation-associated antigen in normal pre-B cells." in: International immunology, Vol. 2, Issue 5, pp. 399-406, 1991 (PubMed).

Adkins, Tidmarsh, Weissman: "Normal thymic cortical epithelial cells developmentally regulate the expression of a B-lineage transformation-associated antigen." in: Immunogenetics, Vol. 27, Issue 3, pp. 180-6, 1988 (PubMed).

Morse, Tidmarsh, Holmes et al.: "Expression of the 6C3 antigen on murine hematopoietic neoplasms. Association with expression of abl, ras, fes, src, erbB, and Cas NS-1 oncogenes but not with myc." in: The Journal of experimental medicine, Vol. 165, Issue 3, pp. 920-5, 1987 (PubMed).

Pillemer, Whitlock, Weissman: "Transformation-associated proteins in murine B-cell lymphomas that are distinct from Abelson virus gene products." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 81, Issue 14, pp. 4434-8, 1984 (PubMed).

Goverman, Brabb, Huseby et al.: "TCR signaling regulates thymic organization: lessons from TCR-transgenic mice." in: Immunology today, Vol. 18, Issue 5, pp. 204-8, 1997 (PubMed).

Lin, Taniuchi, Kitamura et al.: "T and B cell development in BP-1/6C3/aminopeptidase A-deficient mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 10, pp. 4681-7, 1998 (PubMed).