Ly-49C/F/I/H Antikörper (FITC)

Antigen: Ly-49C/F/I/H

Klonalität: Monoklonal (14B11)

Wirt: Hamster

Reaktivität: Maus

Konjugat: FITC

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN156324

Produktbeschreibung

Immunogen: IL-2-activated killer cells (LAK) from C57BL/6 mic

Isotyp: IgG

Klon: 14B11

Beschreibung: The 14B11 antibody reacts with a common epitope of Ly-49C/F/I/H family members also known as Ly-49 inhibitory receptors C, F, and I and activating receptor H. It does not react with other Ly-49 members (i.e., Ly-49A, B, D, and G). Ly-49 is a type-II transmembrane, disulfide-linked homodimer with C-type lectin extracellular domains that binds to MHC class I. Ly-49C/F/I/H is expressed on a subset of NK cells, NK-T cells and some CD8 cells. Ly-49C/F/I, like other members of this family, inhibits NK cell activation through the recognition and interaction of certain MHC class I haplotypes on potential target cells. Ly-49C, Ly-49F, and Ly-49I contain an ITIM motif in their cytoplasmic tails, while Ly-49H lacks ITIM. Ly-49H is encoded by the Cmv1r gene, which promotes resistance to infection by mouse cytomegalovirus (CMV). BALB/c mice are reported to lack the Ly-49H and Ly-49I receptors. The 14B11 antibody, when bound to NK cells, is capable of inducing redirected cell lysis and blocking NK cell effector function. Distribution: NK and NK-T cells, some CD8 T cells Function: NK cell activation or inhibition Synonyms: Ly-49 inhibitory receptors C, F and I and the activating receptor H Structure: Ly-49 family, 110 kD

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: induction of redirected lysis, and blocking of the binding of H-2 lymphoblasts to transfectants expressing Ly-49C, Ly-49F, and Ly-49I. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Reinigung: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Lagerung: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Forschungsgebiet: Zell-/Gewebemarker, Immunologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

C57BL6 mouse splenocytes stained with 14B11 PE and DX5 FITC

Publikationen

Publikationen: Yokoyama, Seaman: "The Ly-49 and NKR-P1 gene families encoding lectin-like receptors on natural killer cells: the NK gene complex." in: Annual review of immunology, Vol. 11, pp. 613-35, 1993 (PubMed).

Ryan, Seaman: "Divergent functions of lectin-like receptors on NK cells." in: Immunological reviews, Vol. 155, pp. 79-89, 1997 (PubMed).

Mason, Gosselin, Anderson et al.: "Differential tyrosine phosphorylation of inhibitory versus activating Ly-49 receptor proteins and their recruitment of SHP-1 phosphatase." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 9, pp. 4187-96, 1997 (PubMed).

Lanier: "NK cell receptors." in: Annual review of immunology, Vol. 16, pp. 359-93, 1998 (PubMed).

Hanke, Takizawa, McMahon et al.: "Direct assessment of MHC class I binding by seven Ly49 inhibitory NK cell receptors." in: Immunity, Vol. 11, Issue 1, pp. 67-77, 1999 (PubMed).

Corral, Takizawa, Hanke et al.: "A new monoclonal antibody reactive with several Ly49 NK cell receptors mediates redirected lysis of target cells." in: Hybridoma, Vol. 18, Issue 4, pp. 359-66, 1999 (PubMed).