I-A/I-E Antikörper (Pacific Blue)

Antigen: I-A/I-E

Klonalität: Monoklonal (M5-114-15-2)

Wirt: Ratte

Reaktivität: Maus

Konjugat: Pacific Blue

Applikation: Durchflusszytometrie (FACS), Intrazelluläre Durchflusszytometrie (ICFC)

Produktnummer: ABIN156289

Produktbeschreibung

Immunogen: Activated C57BL/6 mouse spleen cells

Isotyp: IgG2b, kappa chain

Klon: M5-114-15-2

Beschreibung: The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-A, I-A, I-A, I-E, and I-E MHC class II alloantigens. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2 bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The M5/114.15.2 antibody cross-reacts with the H-2 haplotypes. The M5/114.15.2 antibody has been reported to block antigen presentation. Distribution: B and activated T cells, APCs of the H-2 bearing mice Function: Antigen presentation Synonyms: MHC class II Structure: MHC class II

Spezifität: Reacts with a shared determinant on i-a

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: immunoprecipitation, intracellular immunofluorescent staining, immunohistochemistry of frozen sections, and in vitro and in vivo blocking of antigen presentation or ligand binding. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN156279). This reagent is developed for immunofluorescent staining for flow cytometric analysis, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application. * Pacific Blue(TM) has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue(TM) conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue(TM) is a registered trademark of Molecular Probes, Inc. Pacific Blue(TM) dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.

Reinigung: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue(TM) under optimal conditions. The solution is free of unconjugated Pacific Blue(TM).

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Lagerung: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-I-A/I-E antibody (Pacific Blue)

Publikationen

Publikationen: Bhattacharya, Dorf, Springer: "A shared alloantigenic determinant on Ia antigens encoded by the I-A and I-E subregions: evidence for I region gene duplication." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 127, Issue 6, pp. 2488-95, 1982 (PubMed).

Viville, Neefjes, Lotteau et al.: "Mice lacking the MHC class II-associated invariant chain." in: Cell, Vol. 72, Issue 4, pp. 635-48, 1993 (PubMed).

Yamashita, Nagata, Tada et al.: "CD69 cell surface expression identifies developing thymocytes which audition for T cell antigen receptor-mediated positive selection." in: International immunology, Vol. 5, Issue 9, pp. 1139-50, 1994 (PubMed).

Nelson, Hosier, Brady et al.: "Medullary thymic epithelium expresses a ligand for CTLA4 in situ and in vitro." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 5, pp. 2453-61, 1993 (PubMed).

Watts: "Capture and processing of exogenous antigens for presentation on MHC molecules." in: Annual review of immunology, Vol. 15, pp. 821-50, 1997 (PubMed).

Shi, Kaliyaperumal, Lu et al.: "Promiscuous presentation and recognition of nucleosomal autoepitopes in lupus: role of autoimmune T cell receptor alpha chain." in: The Journal of experimental medicine, Vol. 187, Issue 3, pp. 367-78, 1998 (PubMed).

Pamer, Cresswell: "Mechanisms of MHC class I--restricted antigen processing." in: Annual review of immunology, Vol. 16, pp. 323-58, 1998 (PubMed).

Kim, Noh, Kim et al.: "Activated natural killer cell-mediated immunity is required for the inhibition of tumor metastasis by dendritic cell vaccination." in: Experimental & molecular medicine, Vol. 36, Issue 5, pp. 428-43, 2004 (PubMed).

Luckashenak, Ryszkiewicz, Ramsey et al.: "The Src homology 2 domain-containing leukocyte protein of 76-kDa adaptor links integrin ligation with p44/42 MAPK phosphorylation and podosome distribution in murine dendritic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 8, pp. 5177-85, 2006 (PubMed).