I-A/I-E Antikörper (PE/Cy5)

Antigen: I-A/I-E

Klonalität: Monoklonal (M5-114-15-2)

Wirt: Ratte

Reaktivität: Maus

Konjugat: PE,Cy5

Applikation: Durchflusszytometrie (FACS), Intrazelluläre Durchflusszytometrie (ICFC)

Produktnummer: ABIN156281

Produktbeschreibung

Immunogen: Activated C57BL/6 mouse spleen cells

Isotyp: IgG2b, kappa chain

Klon: M5-114-15-2

Beschreibung: The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-A, I-A, I-A, I-E, and I-E MHC class II alloantigens. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2 bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The M5/114.15.2 antibody cross-reacts with the H-2 haplotypes. The M5/114.15.2 antibody has been reported to block antigen presentation. Distribution: B and activated T cells, APCs of the H-2 bearing mice Function: Antigen presentation Synonyms: MHC class II Structure: MHC class II

Spezifität: Reacts with a shared determinant on i-a

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: immunoprecipitation, intracellular immunofluorescent staining, immunohistochemistry of frozen sections, and in vitro and in vivo blocking of antigen presentation or ligand binding. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN156279). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining,the suggested use of this reagent is < 0.25 µg per 10cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Reinigung: The antibody was purified by affinity chromatography, and conjugated with PE/Cy5 under optimal conditions. The solution is free of unconjugated PE/Cy5 and unconjugated antibody.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Lagerung: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-I-A/I-E antibody (PE/Cy5)

Publikationen

Publikationen: Bhattacharya, Dorf, Springer: "A shared alloantigenic determinant on Ia antigens encoded by the I-A and I-E subregions: evidence for I region gene duplication." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 127, Issue 6, pp. 2488-95, 1982 (PubMed).

Viville, Neefjes, Lotteau et al.: "Mice lacking the MHC class II-associated invariant chain." in: Cell, Vol. 72, Issue 4, pp. 635-48, 1993 (PubMed).

Yamashita, Nagata, Tada et al.: "CD69 cell surface expression identifies developing thymocytes which audition for T cell antigen receptor-mediated positive selection." in: International immunology, Vol. 5, Issue 9, pp. 1139-50, 1994 (PubMed).

Nelson, Hosier, Brady et al.: "Medullary thymic epithelium expresses a ligand for CTLA4 in situ and in vitro." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 5, pp. 2453-61, 1993 (PubMed).

Watts: "Capture and processing of exogenous antigens for presentation on MHC molecules." in: Annual review of immunology, Vol. 15, pp. 821-50, 1997 (PubMed).

Shi, Kaliyaperumal, Lu et al.: "Promiscuous presentation and recognition of nucleosomal autoepitopes in lupus: role of autoimmune T cell receptor alpha chain." in: The Journal of experimental medicine, Vol. 187, Issue 3, pp. 367-78, 1998 (PubMed).

Pamer, Cresswell: "Mechanisms of MHC class I--restricted antigen processing." in: Annual review of immunology, Vol. 16, pp. 323-58, 1998 (PubMed).

Kim, Noh, Kim et al.: "Activated natural killer cell-mediated immunity is required for the inhibition of tumor metastasis by dendritic cell vaccination." in: Experimental & molecular medicine, Vol. 36, Issue 5, pp. 428-43, 2004 (PubMed).

Luckashenak, Ryszkiewicz, Ramsey et al.: "The Src homology 2 domain-containing leukocyte protein of 76-kDa adaptor links integrin ligation with p44/42 MAPK phosphorylation and podosome distribution in murine dendritic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 8, pp. 5177-85, 2006 (PubMed).