FasL (CD178) Antikörper

Antigen: FasL (CD178)

Klonalität: Monoklonal (Kay-10)

Wirt: Maus

Reaktivität: Maus

Applikation: Durchflusszytometrie (FACS), Blockierende Antikörper (Inhibition), Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN156220

Produktbeschreibung

Weitere Bezeichnung: FasL (CD178.1)

Immunogen: C57BL/6 mouse FasL cDNA-transfected L5178Y cells

Format: Low endotoxin (sterile), Low-endotoxin, azide-free (LEAF)

Isotyp: IgG2b, kappa chain  (Passende Sekundärantikörper)

Klon: Kay-10

Beschreibung: CD178.1 is a 40 kD Fas ligand alloantigen (designated as mFasL.1) expressed on the activated T lymphocytes, testis, and eye of selected mouse strains including C57BL/6, C3H, MRL, NOD, NZB, NZW, and SJL. It does not react with lymphoblasts from BALB/c, DBA/1, or DBA/2 mice. CD178.1 is upregulated in activated T cells upon TCR re-engagement and has been shown to induce autocrine and paracrine T cell death. CD178 expression in the eye and testis has been shown to participate in immune privilege at these sites. Functional studies suggest that mFasL.2 has higher specific activity than mFasL.1. CD178 can be cleaved from the surface by metalloproteases, and soluble CD178 may block the activities of membrane-bound CD178. CD178 binds to CD95 (Fas) to induce apoptotic cell death implicated in the maintenance of peripheral tolerance. CD178/CD95 interactions have been implicated in the proliferation of CD8 cells and neutrophil extravasation, chemotaxis, and survival. The Kay-10 antibody has been reported to block CD178.1/CD95 induced apoptosis in cells derived from mice expressing this alloantigen. Distribution: Activated T cells, spleen, testis, eye of some selected mouse strains (C57BL/6, C3H, NZB, NZw, MRL) Function: Induces apoptosis Synonyms: Fas Ligand, FasL, Apo-1 Ligand, CD95 Ligand Structure: TNF superfamily Fas ligand alloantigen, 40 kD

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: in vitro blocking and in vivo blocking, and immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections. Fas Ligand is expressed at low density on activated cells. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (ABIN156218) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (ABIN156216) or biotinylated anti-mouse IgG (ABIN159908) second step , followed by SAv-PE (ABIN159899)). The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN156220). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.25 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Reinheit: LEAF

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.

Lagerung: The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.

Forschungsgebiet: CD Antigene, Apoptose/Nekrose, Virologie, Krebs

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Mouse Fas Ligand transfected cells stained with Kay-10 PE

Publikationen

Publikationen: Takahashi, Tanaka, Brannan et al.: "Generalized lymphoproliferative disease in mice, caused by a point mutation in the Fas ligand." in: Cell, Vol. 76, Issue 6, pp. 969-76, 1994 (PubMed).

Kayagaki, Yamaguchi, Nagao et al.: "Polymorphism of murine Fas ligand that affects the biological activity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 8, pp. 3914-9, 1997 (PubMed).

Kobata, Takasaki, Asahara et al.: "Apoptosis with FasL+ cell infiltration in the periphery and thymus of corrected autoimmune mice." in: Immunology, Vol. 92, Issue 2, pp. 206-13, 1998 (PubMed).

Takeda, Gotoh, Dono et al.: "Protection of islet allografts transplanted together with Fas ligand expressing testicular allografts." in: Diabetologia, Vol. 41, Issue 3, pp. 315-21, 1998 (PubMed).

Hattori, Hirano, Miyajima et al.: "Differential effects of anti-Fas ligand and anti-tumor necrosis factor alpha antibodies on acute graft-versus-host disease pathologies." in: Blood, Vol. 91, Issue 11, pp. 4051-5, 1998 (PubMed).

Hill, Ouhtit, Loughlin et al.: "Fas ligand: a sensor for DNA damage critical in skin cancer etiology." in: Science (New York, N.Y.), Vol. 285, Issue 5429, pp. 898-900, 1999 (PubMed).

Nagata: "Fas ligand-induced apoptosis." in: Annual review of genetics, Vol. 33, pp. 29-55, 2000 (PubMed).

Lee, Lee, Gwak et al.: "Comparisons of CYP2C19 genetic polymorphisms between Korean and Vietnamese populations." in: Therapeutic drug monitoring, Vol. 29, Issue 4, pp. 455-9, 2007 (PubMed).