CD90.2 Antikörper

Antigen: CD90.2

Klonalität: Monoklonal (30-H12)

Wirt: Ratte

Reaktivität: Maus

Applikation: Durchflusszytometrie (FACS), Abreicherung (Depletion), Kostimulation, Immunhistochemie (Gefrierschnitte) (IHC (fro))

Produktnummer: ABIN156086

Produktbeschreibung

Immunogen: Mouse thymus or spleen

Format: Low endotoxin (sterile), Low-endotoxin, azide-free (LEAF)

Isotyp: IgG2b, kappa chain  (Passende Sekundärantikörper)

Klon: 30-H12

Beschreibung: CD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy-1.2. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb/c, CBA/J, C3H/He, C57BL/-, DBA, NZB/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45. The 30-H12 antibody has been reported to induce Ca flux in thymocytes and, in combination with antibody against the CD3/TCR complex, promote thymocyte apoptosis and inhibit CD3-mediated proliferative responses of mature T lymphocytes. Distribution: Hematopoietic stem cells and neurons, all thymocytes, peripheral T cells of the Thy-1.2 bearing mice Function: Lymphocyte costimulation, proliferation and differentiation of hematopoietic stem cells Synonyms: Thy-1.2 Structure: Ig superfamily, 25-35 kD

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: in vivo and in vitro depletion, costimulation of CD3/TCR-mediated signal transduction, and immunohistochemical staining of acetone-fixed frozen sections. The 30-H12 antibody does not react with Thy-1.1 alloantigen of the AKR/J and PL strains. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (ABIN155518/ABIN155519), is recommended prior to immunofluorescent staining. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN156086). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.25 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Reinheit: LEAF

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.

Lagerung: The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.

Forschungsgebiet: CD Antigene

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

C57BL/6 thymocytes stained with 30-H12 FITC

Publikationen

Publikationen: Nakashima, Zhang, Rahman et al.: "Evidence of synergy between Thy-1 and CD3/TCR complex in signal delivery to murine thymocytes for cell death." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 4, pp. 1153-62, 1991 (PubMed).

Ledbetter, Rouse, Micklem et al.: "T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views." in: The Journal of experimental medicine, Vol. 152, Issue 2, pp. 280-95, 1980 (PubMed).

Seaman, Wofsy, Greenspan et al.: "Treatment of autoimmune MRL/Ipr mice with monoclonal antibody to Thy-1.2: a single injection has sustained effects on lymphoproliferation and renal disease." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 130, Issue 4, pp. 1713-8, 1983 (PubMed).

Mayani, Lansdorp: "Thy-1 expression is linked to functional properties of primitive hematopoietic progenitor cells from human umbilical cord blood." in: Blood, Vol. 83, Issue 9, pp. 2410-7, 1994 (PubMed).

Craig, Kay, Cutler et al.: "Expression of Thy-1 on human hematopoietic progenitor cells." in: The Journal of experimental medicine, Vol. 177, Issue 5, pp. 1331-42, 1993 (PubMed).

Nakashima, Pu, Hamaguchi et al.: "Pathway of signal delivery to murine thymocytes triggered by co-crosslinking CD3 and Thy-1 for cellular DNA fragmentation and growth inhibition." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 7, pp. 3511-20, 1993 (PubMed).

Drobyski, Majewski: "Treatment of donor mice with an alpha beta T-cell receptor monoclonal antibody induces prolonged T-cell nonresponsiveness and effectively prevents lethal graft-versus-host disease in murine recipients of major histocompatibility complex (MHC)-matched and " in: Blood, Vol. 87, Issue 12, pp. 5355-69, 1996 (PubMed).

Hardy, Niv, Fadaeev et al.: "BAT mAb induces lymphopoiesis in nude mice." in: International immunology, Vol. 17, Issue 5, pp. 615-9, 2005 (PubMed).

Dyer, Czapiga, Foster et al.: "Eosinophils from lineage-ablated Delta dblGATA bone marrow progenitors: the dblGATA enhancer in the promoter of GATA-1 is not essential for differentiation ex vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 3, pp. 1693-9, 2007 (PubMed).