CD90 (THY1) Antikörper

Antigen: CD90 (THY1)

Synonyme: CD90, FLJ33325

Klonalität: Monoklonal (G7)

Wirt: Ratte

Reaktivität: Maus

Applikation: Durchflusszytometrie (FACS), Aktivierung (Act), Apoptose-Induktion (AI), Immunhistochemie (Gefrierschnitte) (IHC (fro)), Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN156076

Produktbeschreibung

Weitere Bezeichnung: CD90

Immunogen: Mouse T-cell hybridoma C6/G8

Format: Low endotoxin (sterile), Low-endotoxin, azide-free (LEAF)

Isotyp: IgG2c, kappa chain  (Passende Sekundärantikörper)

Klon: G7

Beschreibung: CD90 is a 25-35 kD immunoglobulin superfamily member, also known as Thy-1. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells, but not on B cells. CD90 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90 is involved in costimulation of lymphocyte proliferation and activation, and hematopoietic stem cell differentiation. CD90 has been shown to interact with CD45. The G7 antibody is able to stimulate T cell proliferation and IL-2 secretion and induce apoptosis of thymocytes and CTL clones. Distribution: Hematopoietic stem cells and neurons, all thymocytes, peripheral T cells Function: Lymphocyte costimulation, proliferation and differentiation of hematopoietic stem cells Synonyms: Thy-1, Thy1 Structure: Ig superfamily, 25-35 kD

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: in vitro T-cell activation, induction of apoptosis, and immunohistochemical staining of zinc-fixed paraffin-embedded sections and acetone-fixed frozen sections. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN156076). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.25 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Reinheit: LEAF

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.

Lagerung: The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.

Forschungsgebiet: CD Antigene

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Ucker, Meyers, Obermiller: "Activation-driven T cell death. II. Quantitative differences alone distinguish stimuli triggering nontransformed T cell proliferation or death." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 5, pp. 1583-92, 1992 (PubMed).

Sugiyama, Cantagrel, Reno et al.: "Selection of T cell receptor expression mutants through the functionally linked Ly-6A." in: Cellular immunology, Vol. 130, Issue 2, pp. 271-80, 1990 (PubMed).

Tentori, Pardoll, Zuniga et al.: "Proliferation and production of IL-2 and B cell stimulatory factor 1/IL-4 in early fetal thymocytes by activation through Thy-1 and CD3." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 140, Issue 4, pp. 1089-94, 1988 (PubMed).

Gunter, Malek, Shevach: "T cell-activating properties of an anti-Thy-1 monoclonal antibody. Possible analogy to OKT3/Leu-4." in: The Journal of experimental medicine, Vol. 159, Issue 3, pp. 716-30, 1984 (PubMed).

Mayani, Lansdorp: "Thy-1 expression is linked to functional properties of primitive hematopoietic progenitor cells from human umbilical cord blood." in: Blood, Vol. 83, Issue 9, pp. 2410-7, 1994 (PubMed).

Craig, Kay, Cutler et al.: "Expression of Thy-1 on human hematopoietic progenitor cells." in: The Journal of experimental medicine, Vol. 177, Issue 5, pp. 1331-42, 1993 (PubMed).

Hueber, Raposo, Pierres et al.: "Thy-1 triggers mouse thymocyte apoptosis through a bcl-2-resistant mechanism." in: The Journal of experimental medicine, Vol. 179, Issue 3, pp. 785-96, 1994 (PubMed).