CD80 (CD80) Antikörper (FITC)

Antigen: CD80 (CD80)

Synonyme: LAB7, CD28LG, CD28LG1

Klonalität: Monoklonal (16-10A1)

Wirt: Hamster

Reaktivität: Maus

Konjugat: FITC

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN156005

Produktbeschreibung

Weitere Bezeichnung: CD80

Immunogen: CHO cell line transfected with mouse B7 (CD80)

Isotyp: IgG

Klon: 16-10A1

Beschreibung: CD80 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily, also known as B7-1, B7 and Ly-53. CD80 is constitutively expressed on dendritic cells and monocytes/macrophages, and inducibly expressed on activated B and T cells. The ligation of CD28 on T cells with CD80 and CD86 (B7-2) on antigen presenting cells (such as dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD80 appears to be expressed later in the immune response than CD86. CD80 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells. The 16-10A1 antibody has been shown to block the co-stimulation of T cells by activated B cells in vitro and to block the binding of CTLA-4 to CD80. Distribution: Macrophages, activated B and T cells, dendritic cells Function: T cell costimulation Synonyms: B7-1, B7, Ly-53 Structure: Ig superfamily, 60 kD

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: immunoprecipitation, in vitro and in vivo blocking of CTLA-4 Ig to CD80, and immunohistochemical staining of acetone-fixed frozen sections. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN156010). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.25 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Reinigung: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Lagerung: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Forschungsgebiet: CD Antigene

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

LPS-stimulated (3 days) BALB/c splenocytes stained with 16-10A1 FITC

Publikationen

Publikationen: Razi-Wolf, Freeman, Galvin et al.: "Expression and function of the murine B7 antigen, the major costimulatory molecule expressed by peritoneal exudate cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 9, pp. 4210-4, 1992 (PubMed).

Linsley, Brady, Urnes et al.: "CTLA-4 is a second receptor for the B cell activation antigen B7." in: The Journal of experimental medicine, Vol. 174, Issue 3, pp. 561-9, 1991 (PubMed).

Harlan, Hengartner, Huang et al.: "Mice expressing both B7-1 and viral glycoprotein on pancreatic beta cells along with glycoprotein-specific transgenic T cells develop diabetes due to a breakdown of T-lymphocyte unresponsiveness." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 8, pp. 3137-41, 1994 (PubMed).

Hathcock, Laszlo, Pucillo et al.: "Comparative analysis of B7-1 and B7-2 costimulatory ligands: expression and function." in: The Journal of experimental medicine, Vol. 180, Issue 2, pp. 631-40, 1994 (PubMed).

Herold, Vezys, Koons et al.: "CD28/B7 costimulation regulates autoimmune diabetes induced with multiple low doses of streptozotocin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 2, pp. 984-91, 1997 (PubMed).

Salomon, Bluestone: "Complexities of CD28/B7: CTLA-4 costimulatory pathways in autoimmunity and transplantation." in: Annual review of immunology, Vol. 19, pp. 225-52, 2001 (PubMed).

Andoniou, van Dommelen, Voigt et al.: "Interaction between conventional dendritic cells and natural killer cells is integral to the activation of effective antiviral immunity." in: Nature immunology, Vol. 6, Issue 10, pp. 1011-9, 2005 (PubMed).

Ma, Xu, An et al.: "Vaccine with beta-defensin 2-transduced leukemic cells activates innate and adaptive immunity to elicit potent antileukemia responses." in: Cancer research, Vol. 66, Issue 2, pp. 1169-76, 2006 (PubMed).

Lawson, Manenkova, Ahamed et al.: "Inhibition of transmethylation down-regulates CD4 T cell activation and curtails development of autoimmunity in a model system." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 8, pp. 5366-74, 2007 (PubMed).

Turnquist, Raimondi, Zahorchak et al.: "Rapamycin-conditioned dendritic cells are poor stimulators of allogeneic CD4+ T cells, but enrich for antigen-specific Foxp3+ T regulatory cells and promote organ transplant tolerance." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 11, pp. 7018-31, 2007 (PubMed).