Integrin beta 3 (ITGB3) Antikörper

Antigen: Integrin beta 3 (ITGB3)

Synonyme: LAD, CD18, MF17, MFI7, LCAMB, LFA-1, MAC-1, CD61, GP3A, GPIIIa, INGRB3, MGC159221, MGC159223, integrin, ITGB3

Klonalität: Monoklonal (2C9-G2 (HMb3-1)-2C9-G2 (HMß3-1))

Wirt: Hamster

Reaktivität: Maus, Ratte (Rattus)

Applikation: Durchflusszytometrie (FACS), Blockierende Antikörper (Inhibition), Aktivierung (Act), Immunhistochemie (Gefrierschnitte) (IHC (fro))

Produktnummer: ABIN155944

Produktbeschreibung

Weitere Bezeichnung: CD61

Immunogen: Vitronectin receptor protein from the mouse T-cell hybridoma 2B4

Format: Low endotoxin (sterile), Low-endotoxin, azide-free (LEAF)

Isotyp: IgG  (Passende Sekundärantikörper)

Klon: 2C9-G2 (HMb3-1)-2C9-G2 (HMß3-1)

Beschreibung: CD61 is a 110 kD integrin beta chain also known as beta3 integrin or gpIIIa. It associates with the integrin alphav chain (CD51) to form the vitronectin receptor. In addition, CD61 can associate with the integrin alphaIIb chain (CD41) to form the gpIIb/IIIa complex. CD61 is expressed on platelets, megakaryocytes, endothelium, smooth muscle, a subset of B cells, myeloid cells, osteoclasts, and mast cells. CD61, in conjunction with CD41 or CD51, mediates adhesion to fibronectin, fibrinogen, vitronectin, thrombospondin, and von Willebrand factor. Leukocyte-endothelial adhesion is mediated by the binding of alphav/beta3 integrin or vitronectin receptor to CD31 (PECAM-1). The 2C9.G2 antibody reacts with mouse and rat CD61, and is able to block ligand binding. Distribution: Platelets, megakaryocytes, endothelium, smooth muscle, activated T cells, subset of B cells, monocytes, macrophages, osteoclasts, mast cells Function: Attachment to matrix Synonyms: beta3 integrin, Integrin beta3 chain, gpIIIa Structure: Integrin family, 110 kD

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: blocking of ligand binding, activation of alphav beta3 integrin signaling, and immunohistochemical staining of acetone-fixed frozen sections. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN155944). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Reinheit: LEAF

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.

Lagerung: The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.

Forschungsgebiet: CD Antigene

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

C57BL/6 mouse bone marrow cells stained with HMbeta3-1 FITC

Publikationen

Publikationen: Phillips, Hulbert, Bibby et al.: "In vitro activity of the novel indoloquinone EO-9 and the influence of pH on cytotoxicity." in: British journal of cancer, Vol. 65, Issue 3, pp. 359-64, 1992 (PubMed).

Moulder, Roberts, Shevach et al.: "The mouse vitronectin receptor is a T cell activation antigen." in: The Journal of experimental medicine, Vol. 173, Issue 2, pp. 343-7, 1991 (PubMed).

Kieffer, Phillips: "Platelet membrane glycoproteins: functions in cellular interactions." in: Annual review of cell biology, Vol. 6, pp. 329-57, 1991 (PubMed).

Schultz, Armant: "Beta 1- and beta 3-class integrins mediate fibronectin binding activity at the surface of developing mouse peri-implantation blastocysts. Regulation by ligand-induced mobilization of stored receptor." in: The Journal of biological chemistry, Vol. 270, Issue 19, pp. 11522-31, 1995 (PubMed).

Piali, Hammel, Uherek et al.: "CD31/PECAM-1 is a ligand for alpha v beta 3 integrin involved in adhesion of leukocytes to endothelium." in: The Journal of cell biology, Vol. 130, Issue 2, pp. 451-60, 1995 (PubMed).

Felding-Habermann, Cheresh: "Vitronectin and its receptors." in: Current opinion in cell biology, Vol. 5, Issue 5, pp. 864-8, 1993 (PubMed).

Ashkar, Weber, Panoutsakopoulou et al.: "Eta-1 (osteopontin): an early component of type-1 (cell-mediated) immunity." in: Science (New York, N.Y.), Vol. 287, Issue 5454, pp. 860-4, 2000 (PubMed).