CD48 Molecule (CD48) Antikörper

Antigen: CD48 Molecule (CD48)

Synonyme: BCM1, BLAST, hCD48, mCD48, BLAST1, SLAMF2, MEM-102, Bcm-1, Sgp-60, BLAST-1, AI449234, AW610730

Klonalität: Monoklonal (HM48-1)

Wirt: Hamster

Reaktivität: Maus

Applikation: Durchflusszytometrie (FACS), Immunpräzipitation (IP), Kostimulation, Blockierende Antikörper (Inhibition), Blockierende Antikörper (Inhibition)

Produktnummer: ABIN155858

Produktbeschreibung

Weitere Bezeichnung: CD48

Immunogen: Mouse T lymphoma MBL-2

Format: Low endotoxin (sterile), Low-endotoxin, azide-free (LEAF)

Isotyp: IgG  (Passende Sekundärantikörper)

Klon: HM48-1

Beschreibung: CD48 is a 45 kD GPI-anchored glycoprotein, also known as BCM1, Blast-1 (human), or OX-45 (rat). It is a member of the Ig superfamily, expressed on T and B cells, and monocytes/macrophages. It plays a role in adhesion and T cell recognition. The primary ligands for CD48 are CD2 and CD244. The HM48-1 antibody is useful for blocking in vitro and in vivo CD48 mediated interactions. Distribution: T and B cells, monocytes/macrophages Function: Adhesion, T cell recognition Synonyms: BCM1, Blast-1 (human), OX-45 (rat) Structure: Ig superfamily, 45 kD

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: immunoprecipitation, costimulation of T cell proliferation, blocking of CD48-CD2 interaction, and inhibition of CTL activity and graft rejection. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN155858). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.25 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Reinheit: LEAF

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.

Lagerung: The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.

Forschungsgebiet: CD Antigene

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

C57BL/6 splenocytes stained with HM48-1 FITC

Publikationen

Publikationen: Kato, Koyanagi, Okada et al.: "CD48 is a counter-receptor for mouse CD2 and is involved in T cell activation." in: The Journal of experimental medicine, Vol. 176, Issue 5, pp. 1241-9, 1992 (PubMed).

Qin, Chavin, Lin et al.: "Anti-CD2 receptor and anti-CD2 ligand (CD48) antibodies synergize to prolong allograft survival." in: The Journal of experimental medicine, Vol. 179, Issue 1, pp. 341-6, 1994 (PubMed).

Flament, Bellagha, Rosenthal-Allieri et al.: "CD48 may serve as an accessory molecule for the activation of a subset of human gamma/delta T cells." in: Human immunology, Vol. 46, Issue 2, pp. 82-92, 1997 (PubMed).

Latchman, McKay, Reiser: "Identification of the 2B4 molecule as a counter-receptor for CD48." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 11, pp. 5809-12, 1998 (PubMed).