CD45RB (CD45RB) Antikörper

Antigen: CD45RB (CD45RB)

Klonalität: Monoklonal (C363-16A)

Wirt: Ratte

Reaktivität: Maus

Applikation: Durchflusszytometrie (FACS), Immunpräzipitation (IP), Immunhistochemie (Gefrierschnitte) (IHC (fro)), Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN155841

Produktbeschreibung

Weitere Bezeichnung: CD45RB

Immunogen: Cloned mouse Th2 cell lines

Format: Purified

Isotyp: IgG2a, kappa chain  (Passende Sekundärantikörper)

Klon: C363-16A

Beschreibung: CD45RB is an isoform of CD45 with exon 5 splicing (encodes B determinant). It is a 220 kD glycoprotein expressed on peripheral B cells, naïve T cells, thymocytes, weakly on macrophages, and dendritic cells. It plays a critical role in TCR and BCR signaling. As T cells become activated and progress from naïve to memory cells, CD45RB expression is downregulated. Additionally, functionally distinct CD4 T cell subsets, which secrete differing cytokine profiles, can be separated by CD45RB intensity. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4 and Thy-1. Distribution: Peripheral B cells, naïve T cells, most thymocytes, low density on macrophages and dendritic cells Function: Phosphatase, T cell development and activation Structure: Phosphatase receptor (CD45 gene exon 5), 220 kD

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.25 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Lagerung: The antibody solution should be stored undiluted at 4 °C.

Forschungsgebiet: CD Antigene

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

C57BL/6 splenocytes stained with C363-16A PE

Publikationen

Publikationen: Pulido, Cebrian, Acevedo et al.: "Comparative biochemical and tissue distribution study of four distinct CD45 antigen specificities." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 140, Issue 11, pp. 3851-7, 1988 (PubMed).

Bottomly, Luqman, Greenbaum et al.: "A monoclonal antibody to murine CD45R distinguishes CD4 T cell populations that produce different cytokines." in: European journal of immunology, Vol. 19, Issue 4, pp. 617-23, 1989 (PubMed).

Trowbridge, Thomas: "CD45: an emerging role as a protein tyrosine phosphatase required for lymphocyte activation and development." in: Annual review of immunology, Vol. 12, pp. 85-116, 1994 (PubMed).

Kishihara, Penninger, Wallace et al.: "Normal B lymphocyte development but impaired T cell maturation in CD45-exon6 protein tyrosine phosphatase-deficient mice." in: Cell, Vol. 74, Issue 1, pp. 143-56, 1993 (PubMed).

Norian, Allen: "No intrinsic deficiencies in CD8+ T cell-mediated antitumor immunity with aging." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 2, pp. 835-44, 2004 (PubMed).