Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) Antikörper

Antigen: Protein tyrosine Phosphatase, Receptor Type, C (PTPRC)

Synonyme: LCA, LY5, B220, CD45, T200, CD45R, GP180, Lca, RT7, L-CA, PTPRC, si:dkeyp-47c5.1

Klonalität: Monoklonal (RA3-6B2)

Wirt: Ratte

Reaktivität: Maus

Applikation: Durchflusszytometrie (FACS), Immunpräzipitation (IP), Aktivierung (Act), Immunhistochemie (Gefrierschnitte) (IHC (fro)), Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN155823

Produktbeschreibung

Weitere Bezeichnung: CD45R/B220

Immunogen: Abelson murine leukemia virus-induced pre-B tumor cells

Format: Low endotoxin (sterile), Low-endotoxin, azide-free (LEAF)

Isotyp: IgG2a, kappa chain  (Passende Sekundärantikörper)

Klon: RA3-6B2

Beschreibung: CD45R is an isoform of CD45, also known as B220. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Fas and MRL-Fas mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker, however, CD19 may be more appropriate for B cell specificity. Distribution: B cells, T cell subset, NK cell subset Function: Phosphatase, T and B cell activation Synonyms: B220 Structure: Protein tyrosine phosphatase (PTP) family, 180-240 kD

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: immunoprecipitation, in vitro and in vivo modulation of B cell responses, and immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN155823). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.25 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Reinheit: LEAF

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.

Lagerung: The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.

Forschungsgebiet: CD Antigene

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

C57BL/6 splenocytes stained with RA3-6B2 PE

Publikationen

Publikationen: Pulido, Cebrian, Acevedo et al.: "Comparative biochemical and tissue distribution study of four distinct CD45 antigen specificities." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 140, Issue 11, pp. 3851-7, 1988 (PubMed).

Asensi, Kimeno, Kawamura et al.: "Treatment of autoimmune MRL/lpr mice with anti-B220 monoclonal antibody reduces the level of anti-DNA antibodies and lymphadenopathies." in: Immunology, Vol. 68, Issue 2, pp. 204-8, 1989 (PubMed).

Coffman: "Surface antigen expression and immunoglobulin gene rearrangement during mouse pre-B cell development." in: Immunological reviews, Vol. 69, pp. 5-23, 1983 (PubMed).

Domiati-Saad, Ogle, Justement: "Administration of anti-CD45 mAb specific for a B cell-restricted epitope abrogates the B cell response to a T-dependent antigen in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 11, pp. 5936-47, 1994 (PubMed).

Trowbridge, Thomas: "CD45: an emerging role as a protein tyrosine phosphatase required for lymphocyte activation and development." in: Annual review of immunology, Vol. 12, pp. 85-116, 1994 (PubMed).

George, Rath, Shroff et al.: "Ligation of CD45 on B cells can facilitate production of secondary Ig isotypes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 3, pp. 1014-21, 1994 (PubMed).

Kishihara, Penninger, Wallace et al.: "Normal B lymphocyte development but impaired T cell maturation in CD45-exon6 protein tyrosine phosphatase-deficient mice." in: Cell, Vol. 74, Issue 1, pp. 143-56, 1993 (PubMed).

Monteith, Chelack, Davis et al.: "Identification of monoclonal antibodies for immunohistochemical staining of feline B lymphocytes in frozen and formalin-fixed paraffin-embedded tissues." in: Canadian journal of veterinary research = Revue canadienne de recherche vétérinaire, Vol. 60, Issue 3, pp. 193-8, 1996 (PubMed).

Hata, Sakaguchi, Yoshitomi et al.: "Distinct contribution of IL-6, TNF-alpha, IL-1, and IL-10 to T cell-mediated spontaneous autoimmune arthritis in mice." in: The Journal of clinical investigation, Vol. 114, Issue 4, pp. 582-8, 2004 (PubMed).

Shih, Racz, Allen: "Differential MHC class II presentation of a pathogenic autoantigen during health and disease." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 6, pp. 3438-48, 2006 (PubMed).

Chang, Chang, Lee et al.: "Cytopiloyne, a polyacetylenic glucoside, prevents type 1 diabetes in nonobese diabetic mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 11, pp. 6984-93, 2007 (PubMed).

Fazilleau, Eisenbraun, Malherbe et al.: "Lymphoid reservoirs of antigen-specific memory T helper cells." in: Nature immunology, Vol. 8, Issue 7, pp. 753-61, 2007 (PubMed).