CD28 (CD28) Antikörper

Antigen: CD28 (CD28)

Synonyme: Tp44, MGC138290

Klonalität: Monoklonal (37-37-51)

Wirt: Hamster

Reaktivität: Maus

Applikation: Durchflusszytometrie (FACS), Immunpräzipitation (IP), Immunhistochemie (Gefrierschnitte) (IHC (fro)), Kostimulation, Blockierende Antikörper (Inhibition)

Produktnummer: ABIN155631

Produktbeschreibung

Weitere Bezeichnung: CD28

Immunogen: C57BL/6 mouse T-cell lymphoma EL-4

Format: Low endotoxin (sterile), Low-endotoxin, azide-free (LEAF)

Isotyp: IgG  (Passende Sekundärantikörper)

Klon: 37-37-51

Beschreibung: CD28 is a 44 kD glycoprotein, also known as Tp44 or T44. It is a member of the Ig superfamily, expressed on thymocytes, most peripheral T cells, and NK cells. In association with CD80 (B7-1) and CD86 (B7-2), CD28 acts as the second signal for T and NK cell activation and proliferation. The 37.51 antibody has been reported to augment in vitro T cell proliferation and cytokine production, and promote CTL development. Distribution: Thymocytes, CD4, CD8 peripheral T cells, NK cells Function: Costimulates T and NK cells Synonyms: Tp44, T44 Structure: Ig superfamily, 44 kD

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: immunoprecipitation, in vitro costimulation of T and NK cells, in vitro blocking of allogeneic mixed leukocyte response and inhibition of MHC-unrestricted CTL cytotoxicity, in vitro induction of thymocyte differentiation, and immunohistochemical staining of acetone-fixed frozen sections. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN155631). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Reinheit: LEAF

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.

Lagerung: The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.

Forschungsgebiet: CD Antigene

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

C57BL/6 splenocytes stained with 37.51 PE and CD3 FITC

Publikationen

Publikationen: Gross, Michl: "Re: Functional characteristics of sperm obtained by electroejaculation." in: The Journal of urology, Vol. 149, Issue 2, pp. 380, 1993 (PubMed).

Lenschow, Walunas, Bluestone: "CD28/B7 system of T cell costimulation." in: Annual review of immunology, Vol. 14, pp. 233-58, 1996 (PubMed).

Nishio, Spielman, Lee et al.: "CD80 (B7.1) and CD54 (intracellular adhesion molecule-1) induce target cell susceptibility to promiscuous cytotoxic T cell lysis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 10, pp. 4347-53, 1996 (PubMed).

Masten, Yates, Pollard Koga et al.: "Characterization of accessory molecules in murine lung dendritic cell function: roles for CD80, CD86, CD54, and CD40L." in: American journal of respiratory cell and molecular biology, Vol. 16, Issue 3, pp. 335-42, 1997 (PubMed).

Cibotti, Punt, Dash et al.: "Surface molecules that drive T cell development in vitro in the absence of thymic epithelium and in the absence of lineage-specific signals." in: Immunity, Vol. 6, Issue 3, pp. 245-55, 1997 (PubMed).

Zhang, He: "An essential role for c-FLIP in the efficient development of mature T lymphocytes." in: The Journal of experimental medicine, Vol. 202, Issue 3, pp. 395-404, 2005 (PubMed).

Terrazas, Montero, Terrazas et al.: "Role of the programmed Death-1 pathway in the suppressive activity of alternatively activated macrophages in experimental cysticercosis." in: International journal for parasitology, Vol. 35, Issue 13, pp. 1349-58, 2005 (PubMed).

Perchonock, Fernando, Quinn et al.: "Negative regulation of interleukin-2 and p38 mitogen-activated protein kinase during T-cell activation by the adaptor ALX." in: Molecular and cellular biology, Vol. 26, Issue 16, pp. 6005-15, 2006 (PubMed).

Pua, Dzhagalov, Chuck et al.: "A critical role for the autophagy gene Atg5 in T cell survival and proliferation." in: The Journal of experimental medicine, Vol. 204, Issue 1, pp. 25-31, 2007 (PubMed).

Wang, Huang, Goetzl: "Type 1 sphingosine 1-phosphate G protein-coupled receptor (S1P1) mediation of enhanced IL-4 generation by CD4 T cells from S1P1 transgenic mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 8, pp. 4885-90, 2007 (PubMed).

Perchonock, Pajerowski, Nguyen et al.: "The related adaptors, adaptor in lymphocytes of unknown function X and Rlk/Itk-binding protein, have nonredundant functions in lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 3, pp. 1768-75, 2007 (PubMed).