CD25 Antikörper

Antigen: CD25

Klonalität: Monoklonal (PC61)

Wirt: Ratte

Reaktivität: Maus

Applikation: Durchflusszytometrie (FACS), Immunpräzipitation (IP), Immunhistochemie (Gefrierschnitte) (IHC (fro)), Blockierende Antikörper (Inhibition), Abreicherung (Depletion)

Produktnummer: ABIN155607

Produktbeschreibung

Immunogen: IL-2-dependent cytolytic mouse T-cell clone B6.1

Format: Low endotoxin (sterile), Low-endotoxin, azide-free (LEAF)

Isotyp: IgG1, lambda chain  (Passende Sekundärantikörper)

Klon: PC61

Beschreibung: CD25 is a 55 kD glycoprotein, also known as the low affinity IL-2Ralpha, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subset, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rbeta) and CD132 (common gamma chain), CD25 forms the high affinity signaling IL-2 receptor. The PC61 antibody is able to block IL-12 binding to its receptor and inhibit IL-2 stimulated cell growth. Distribution: Activated T and B cells, thymocyte subset, pre-B cells, T regulatory cells Function: IL-2 receptor Synonyms: IL-2Ralpha, Ly-43, p55, Tac Structure: Forms high affinity IL-2R with IL-2Rbeta (CD122) and IL-2Rgamma (CD132), 55 kD

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: immunoprecipitation, in vitro blocking of IL-2 binding to the low- and high-affinity receptors, growth inhibition of IL-2-dependent T-cell lines, in vivo depletion of CD25CD4 Treg cells, and immunohistochemical staining of acetone-fixed frozen sections. PC61 antibody recognizes different epitope of 3C7 antibody (ABIN155589). The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN155607). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.25 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Reinheit: LEAF

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.

Lagerung: The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.

Forschungsgebiet: CD Antigene

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Con A-stimulated (3 days) BALB/c mouse splenocytes stained with LEAF(TM) purified PC61, followed by anti-rat IgG FITC

Publikationen

Publikationen: Waldmann: "The interleukin-2 receptor." in: The Journal of biological chemistry, Vol. 266, Issue 5, pp. 2681-4, 1991 (PubMed).

Moreau, Nabholz, Diamantstein et al.: "Monoclonal antibodies identify three epitope clusters on the mouse p55 subunit of the interleukin 2 receptor: relationship to the interleukin 2-binding site." in: European journal of immunology, Vol. 17, Issue 7, pp. 929-35, 1987 (PubMed).

Cayrol, Clerc, Bertrand et al.: in: (PubMed).

Lowenthal, Zubler, Nabholz et al.: "Similarities between interleukin-2 receptor number and affinity on activated B and T lymphocytes." in: Nature, Vol. 315, Issue 6021, pp. 669-72, 1985 (PubMed).

Lowenthal, Corthesy, Tougne et al.: "High and low affinity IL 2 receptors: analysis by IL 2 dissociation rate and reactivity with monoclonal anti-receptor antibody PC61." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 135, Issue 6, pp. 3988-94, 1985 (PubMed).

Taniguchi, Minami: "The IL-2/IL-2 receptor system: a current overview." in: Cell, Vol. 73, Issue 1, pp. 5-8, 1993 (PubMed).

Onizuka, Tawara, Shimizu et al.: "Tumor rejection by in vivo administration of anti-CD25 (interleukin-2 receptor alpha) monoclonal antibody." in: Cancer research, Vol. 59, Issue 13, pp. 3128-33, 1999 (PubMed).

Read, Malmstroem, Powrie: "Cytotoxic T lymphocyte-associated antigen 4 plays an essential role in the function of CD25(+)CD4(+) regulatory cells that control intestinal inflammation." in: The Journal of experimental medicine, Vol. 192, Issue 2, pp. 295-302, 2000 (PubMed).

Takahashi, Tagami, Yamazaki et al.: "Immunologic self-tolerance maintained by CD25(+)CD4(+) regulatory T cells constitutively expressing cytotoxic T lymphocyte-associated antigen 4." in: The Journal of experimental medicine, Vol. 192, Issue 2, pp. 303-10, 2000 (PubMed).

Pasare, Medzhitov: "Toll-dependent control mechanisms of CD4 T cell activation." in: Immunity, Vol. 21, Issue 5, pp. 733-41, 2004 (PubMed).

Lei, Scott: "Induction of tolerance to factor VIII inhibitors by gene therapy with immunodominant A2 and C2 domains presented by B cells as Ig fusion proteins." in: Blood, Vol. 105, Issue 12, pp. 4865-70, 2005 (PubMed).

Le, Thomas, Ecochard et al.: "Proportion of long-term event-free survivors and lifetime of adult patients not cured after a standard acute lymphoblastic leukemia therapeutic program: adult acute lymphoblastic leukemia-94 trial." in: Cancer, Vol. 109, Issue 10, pp. 2058-67, 2007 (PubMed).

Cao, Dobrzynski, Wang et al.: "Induction and role of regulatory CD4+CD25+ T cells in tolerance to the transgene product following hepatic in vivo gene transfer." in: Blood, Vol. 110, Issue 4, pp. 1132-40, 2007 (PubMed).

Benson, Pino-Lagos, Rosemblatt et al.: "All-trans retinoic acid mediates enhanced T reg cell growth, differentiation, and gut homing in the face of high levels of co-stimulation." in: The Journal of experimental medicine, Vol. 204, Issue 8, pp. 1765-74, 2007 (PubMed).