CD24 Molecule (CD24) Antikörper

Antigen: CD24 Molecule (CD24)

Synonyme: HSA, Cd24, Ly-52, nectadrin, CD24A, FLJ22950, FLJ43543, MGC75043

Klonalität: Monoklonal (M1-69)

Wirt: Ratte

Reaktivität: Maus

Applikation: Durchflusszytometrie (FACS), Western Blot (WB), Immunhistochemie (Gefrierschnitte) (IHC (fro)), Immunhistochemie (Paraffinschnitte) (IHC (p)), Apoptose-Induktion (AI)

Produktnummer: ABIN155575

Produktbeschreibung

Weitere Bezeichnung: CD24

Immunogen: C57BL/10 mouse splenic T cells and concanavalin A-activated splenocytes

Format: Low endotoxin (sterile), Low-endotoxin, azide-free (LEAF)

Isotyp: IgG2b, kappa chain  (Passende Sekundärantikörper)

Klon: M1-69

Beschreibung: CD24 is a 35-45 kD protein, also known as Heat Stable Antigen (HSA), Ly-52, or Nectadrin. It is a GPI-linked sialoglycoprotein expressed on lymphocytes, granulocytes, epithelial cells, thymocytes, monocytes, erythrocytes, and dendritic cells. CD24 expression varies during T and B cell differentiation and is a useful marker for delineating various lymphocyte developmental stages. CD24 serves as an adhesion or costimulatory molecule involved in T and B lymphocyte activation and differentiation by homophilic binding or binding to CD62P. Distribution: B cells, granulyocytes, epithelial cells, thymocytes, monocytes, erythrocytes, dendritic cells Function: Regulates B cell proliferation and differentiation Synonyms: Heat Stable Antigen (HSA), Ly-52, Nectadrin Structure: GPI-linked sialoglycoprotein, 35-45 kD

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: Western blotting, in vitro induction of thymocyte maturation, complement-mediated cytotoxicity, and immunohistochemistry of acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN155575). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.25 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Reinheit: LEAF

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.

Lagerung: The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.

Forschungsgebiet: CD Antigene

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

C57BL/6 mouse splenocytes stained with LEAF(TM) purified M1/69, followed by anti-rat IgG FITC

Publikationen

Publikationen: Springer, Galfre, Secher et al.: "Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens." in: European journal of immunology, Vol. 8, Issue 8, pp. 539-51, 1978 (PubMed).

Liu, Jones, Aruffo et al.: "Heat-stable antigen is a costimulatory molecule for CD4 T cell growth." in: The Journal of experimental medicine, Vol. 175, Issue 2, pp. 437-45, 1992 (PubMed).

Veillette, Zuniga-Pfluecker, Bolen et al.: "Engagement of CD4 and CD8 expressed on immature thymocytes induces activation of intracellular tyrosine phosphorylation pathways." in: The Journal of experimental medicine, Vol. 170, Issue 5, pp. 1671-80, 1989 (PubMed).

Crowley, Inaba, Witmer-Pack et al.: "The cell surface of mouse dendritic cells: FACS analyses of dendritic cells from different tissues including thymus." in: Cellular immunology, Vol. 118, Issue 1, pp. 108-25, 1989 (PubMed).

Hough, Chappel, Sauvageau et al.: "Reduction of early B lymphocyte precursors in transgenic mice overexpressing the murine heat-stable antigen." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 2, pp. 479-88, 1996 (PubMed).

Aigner, Sthoeger, Fogel et al.: "CD24, a mucin-type glycoprotein, is a ligand for P-selectin on human tumor cells." in: Blood, Vol. 89, Issue 9, pp. 3385-95, 1997 (PubMed).

Chappaz, Flueck, Farr et al.: "Increased TSLP availability restores T- and B-cell compartments in adult IL-7 deficient mice." in: Blood, Vol. 110, Issue 12, pp. 3862-70, 2007 (PubMed).