CD8a Antikörper (FITC)

Antigen: CD8a

Klonalität: Monoklonal (53-6-7)

Wirt: Ratte

Reaktivität: Maus

Konjugat: FITC

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN155440

Produktbeschreibung

Immunogen: Mouse thymus or spleen

Isotyp: IgG2a, kappa chain

Klon: 53-6-7

Beschreibung: CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked alpha and beta chains that form the alpha(CD8a)/beta(CD8b) heterodimer and alpha/alpha homodimer. CD8a is a 34kD protein that belongs to the immunoglobulin family. The CD8 alpha/beta heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR alpha/beta T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 alpha/alpha homodimer is expressed on a subset of gamma/delta TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a cells. Distribution: Most thymocytes, T cell subset, some NK cells Function: Co-receptor for TCR Synonyms: T8, Lyt2, Ly-2 Structure: Ig superfamily, CD8alpha chain, 34 kD

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: immunoprecipitation, in vivo and in vitro cell depletion, inhibition of CD8 T cell proliferation, blocking of cytotoxicity, and immunohistochemical staining of acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and 10% formalin-fixed paraffin-embedded sections. Special Note: The antibody works well on acetone-fixed frozen sections as well as Zinc-fixed paraffin-embedded sections. It sometime works on formalin-fixed paraffin-embedded tissue sections but inconsistent results have been reported. The 53-6.7 antibody competes with 5H10-1 antibody (ABIN155467) for binding to thymocytes. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN155451). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.25 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Reinigung: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Lagerung: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Forschungsgebiet: CD Antigene

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

C57BL/6 splenocytes stained with 53-6.7 FITC and CD3 PE

Publikationen

Publikationen: Ledbetter, Herzenberg: "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens." in: Immunological reviews, Vol. 47, pp. 63-90, 1980 (PubMed).

Takahashi, Nakata, Tanaka et al.: "CD4 and CD8 regulate interleukin 2 responses of T cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 12, pp. 5557-61, 1992 (PubMed).

Ledbetter, Seaman, Tsu et al.: "Lyt-2 and lyt-3 antigens are on two different polypeptide subunits linked by disulfide bonds. Relationship of subunits to T cell cytolytic activity." in: The Journal of experimental medicine, Vol. 153, Issue 6, pp. 1503-16, 1981 (PubMed).

Zamoyska: "The CD8 coreceptor revisited: one chain good, two chains better." in: Immunity, Vol. 1, Issue 4, pp. 243-6, 1995 (PubMed).

Ellmeier, Sawada, Littman: "The regulation of CD4 and CD8 coreceptor gene expression during T cell development." in: Annual review of immunology, Vol. 17, pp. 523-54, 1999 (PubMed).

Fan, Kouda, Nakamura et al.: "Effects of dietary restriction on spontaneous dermatitis in NC/Nga mice." in: Experimental biology and medicine (Maywood, N.J.), Vol. 226, Issue 11, pp. 1045-50, 2001 (PubMed).

Hata, Sakaguchi, Yoshitomi et al.: "Distinct contribution of IL-6, TNF-alpha, IL-1, and IL-10 to T cell-mediated spontaneous autoimmune arthritis in mice." in: The Journal of clinical investigation, Vol. 114, Issue 4, pp. 582-8, 2004 (PubMed).

Ko, Ko, Chang et al.: "alpha-Galactosylceramide can act as a nasal vaccine adjuvant inducing protective immune responses against viral infection and tumor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 5, pp. 3309-17, 2005 (PubMed).

Shih, Racz, Allen: "Differential MHC class II presentation of a pathogenic autoantigen during health and disease." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 6, pp. 3438-48, 2006 (PubMed).

Bouwer, Alberti-Segui, Montfort et al.: "Directed antigen delivery as a vaccine strategy for an intracellular bacterial pathogen." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 13, pp. 5102-7, 2006 (PubMed).

Kamimura, Sawa, Sato et al.: "IL-2 in vivo activities and antitumor efficacy enhanced by an anti-IL-2 mAb." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 1, pp. 306-14, 2006 (PubMed).