CD4 Molecule (CD4) Antikörper

Antigen: CD4 Molecule (CD4)

Synonyme: CD4, MGC165891, cd4, CD4mut, L3T4, Ly-4, p55, W3/25

Klonalität: Monoklonal (GK1-5)

Wirt: Ratte

Reaktivität: Maus

Applikation: Durchflusszytometrie (FACS), Blockierende Antikörper (Inhibition), Kostimulation, Abreicherung (Depletion), Immunhistochemie (Gefrierschnitte) (IHC (fro)), Immunpräzipitation (IP)

Produktnummer: ABIN155377

Produktbeschreibung

Weitere Bezeichnung: CD4

Immunogen: Mouse CTL clone V4

Format: Low endotoxin (sterile), Low-endotoxin, azide-free (LEAF)

Isotyp: IgG2b, kappa chain  (Passende Sekundärantikörper)

Klon: GK1-5

Beschreibung: CD4 is a 55 kD protein, also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosin kinase, lck. The GK1.5 antibody is able to block CD4 mediated cell adhesion and T cell activation. It is also useful for in vivo and in vitro depletion of CD4 T cells. Distribution: Majority of thymocytes, T cell subset Function: TCR co-receptor, T cell activation Synonyms: L3T4, T4 Structure: Ig superfamily, 55 kD

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: blocking of CD4 T cell activation, thymocyte costimulation, in vitro and in vivo depletion, blocking of egg-cell to sperm cell adhesion, immunohistochemical staining of acetone-fixed frozen sections, and immunoprecipitation. Binding of GK1.5 antibody to CD4 T cells can be blocked by RM4-5 antibody (ABIN155393), but not RM4-4 antibody (ABIN156802). The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN155377). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.25 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Reinheit: LEAF

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.

Lagerung: The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.

Forschungsgebiet: CD Antigene

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

C57BL/6 mouse splenocytes stained with LEAF(TM) purified GK1.5, followed by anti-rat IgG FITC

Publikationen

Publikationen: Janeway: "The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation." in: Annual review of immunology, Vol. 10, pp. 645-74, 1992 (PubMed).

Wu, Antica, Johnson et al.: "Developmental potential of the earliest precursor cells from the adult mouse thymus." in: The Journal of experimental medicine, Vol. 174, Issue 6, pp. 1617-27, 1992 (PubMed).

Bierer, Sleckman, Ratnofsky et al.: "The biologic roles of CD2, CD4, and CD8 in T-cell activation." in: Annual review of immunology, Vol. 7, pp. 579-99, 1989 (PubMed).

Israelski, Araujo, Conley et al.: "Treatment with anti-L3T4 (CD4) monoclonal antibody reduces the inflammatory response in toxoplasmic encephalitis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 3, pp. 954-8, 1989 (PubMed).

Ghobrial, Boublik, Winn et al.: "In vivo use of monoclonal antibodies against murine T cell antigens." in: Clinical immunology and immunopathology, Vol. 52, Issue 3, pp. 486-506, 1989 (PubMed).

Dialynas, Wilde, Marrack et al.: "Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with class II MHC antigen-reactivity." in: Immunological reviews, Vol. 74, pp. 29-56, 1983 (PubMed).

Dialynas, Quan, Wall et al.: "Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 131, Issue 5, pp. 2445-51, 1983 (PubMed).

Godfrey, Kennedy, Mombaerts et al.: "Onset of TCR-beta gene rearrangement and role of TCR-beta expression during CD3-CD4-CD8- thymocyte differentiation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 10, pp. 4783-92, 1994 (PubMed).

Schuyler, Gott, Edwards et al.: "Experimental hypersensitivity pneumonitis. Effect of CD4 cell depletion." in: American journal of respiratory and critical care medicine, Vol. 149, Issue 5, pp. 1286-94, 1994 (PubMed).

Gavett, Chen, Finkelman et al.: "Depletion of murine CD4+ T lymphocytes prevents antigen-induced airway hyperreactivity and pulmonary eosinophilia." in: American journal of respiratory cell and molecular biology, Vol. 10, Issue 6, pp. 587-93, 1994 (PubMed).

Zheng, Han, Kelsoe: "T helper cells in murine germinal centers are antigen-specific emigrants that downregulate Thy-1." in: The Journal of experimental medicine, Vol. 184, Issue 3, pp. 1083-91, 1997 (PubMed).

Frei, Eugster, Bopst et al.: "Tumor necrosis factor alpha and lymphotoxin alpha are not required for induction of acute experimental autoimmune encephalomyelitis." in: The Journal of experimental medicine, Vol. 185, Issue 12, pp. 2177-82, 1997 (PubMed).

Felix, Donermeyer, Horvath et al.: "Alloreactive T cells respond specifically to multiple distinct peptide-MHC complexes." in: Nature immunology, Vol. 8, Issue 4, pp. 388-97, 2007 (PubMed).