CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antikörper

Antigen: CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E)

Synonyme: T3E, TCRE, FLJ18683, CD3E, cd3e, CD3, t3e, tcre

Klonalität: Monoklonal (145-2C11)

Wirt: Hamster

Reaktivität: Maus

Applikation: Durchflusszytometrie (FACS), Immunhistochemie (Gefrierschnitte) (IHC (fro)), Immunhistochemie (Paraffinschnitte) (IHC (p)), Immunpräzipitation (IP), Western Blot (WB), Blockierende Antikörper (Inhibition), Apoptose-Induktion (AI), Abreicherung (Depletion), Aktivierung (Act)

Produktnummer: ABIN155351

Produktbeschreibung

Weitere Bezeichnung: CD3epsilon

Immunogen: H-2K-specific mouse cytotoxic T lymphocyte clone BM10-37

Format: Low endotoxin (sterile), Low-endotoxin, azide-free (LEAF)

Isotyp: IgG  (Passende Sekundärantikörper)

Klon: 145-2C11

Beschreibung: CD3epsilon is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3epsilon forms a TCR complex by associating with the CD3delta, gamma and zeta chains, as well as the TCR alpha/beta or gamma/delta chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex. The 145-2C11 antibody is useful for in vitro blocking and activation assays, as well as apoptosis induction and in vivo T cell depletions. Distribution: Thymocytes (differentiation dependent), mature T cells, NK-T cells Function: TCR signal transduction, T cell activation, antigen recognition Synonyms: CD3epsilon, T3, CD3 Structure: Ig superfamily, forms CD3/TCR complex with CD3delta, gamma and zeta subunits and TCR (alpha/beta and gamma/delta), 20 kD

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting, complement-mediated cytotoxicity, in vitro and in vivo stimulation of T cells, and in vivo T cell depletions. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific of T cells. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN155351). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.25 µg per 10 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.

Reinheit: LEAF

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.

Lagerung: The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.

Forschungsgebiet: CD Antigene

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

C57BL/6 mouse splenocytes stained with LEAF(TM) purified 145-2C11, followed by anti-Armenian hamster IgG FITC

Publikationen

Publikationen: Payer, Elbe, Stingl: "Circulating CD3+/T cell receptor V gamma 3+ fetal murine thymocytes home to the skin and give rise to proliferating dendritic epidermal T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 8, pp. 2536-43, 1991 (PubMed).

Davis: "T cell receptor gene diversity and selection." in: Annual review of biochemistry, Vol. 59, pp. 475-96, 1990 (PubMed).

Miescher, Schreyer, MacDonald: "Production and characterization of a rat monoclonal antibody against the murine CD3 molecular complex." in: Immunology letters, Vol. 23, Issue 2, pp. 113-8, 1990 (PubMed).

Leo, Foo, Sachs et al.: "Identification of a monoclonal antibody specific for a murine T3 polypeptide." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 5, pp. 1374-8, 1987 (PubMed).

Henrickson, Reid, Bellet et al.: "Comparison of in vivo efficacy and mechanism of action of antimurine monoclonal antibodies directed against TCR alpha beta (H57-597) and CD3 (145-2C11)." in: Transplantation, Vol. 60, Issue 8, pp. 828-35, 1995 (PubMed).

Vossen, Tibbe, Kroos et al.: "Fc receptor binding of anti-CD3 monoclonal antibodies is not essential for immunosuppression, but triggers cytokine-related side effects." in: European journal of immunology, Vol. 25, Issue 6, pp. 1492-6, 1995 (PubMed).

Salvadori, Gansbacher, Pizzimenti et al.: "Abnormal signal transduction by T cells of mice with parental tumors is not seen in mice bearing IL-2-secreting tumors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 11, pp. 5176-82, 1994 (PubMed).

Jacobs, Vandeputte, Tolkamp et al.: "CD3 components at the surface of pro-T cells can mediate pre-T cell development in vivo." in: European journal of immunology, Vol. 24, Issue 4, pp. 934-9, 1994 (PubMed).

Weiss, Littman: "Signal transduction by lymphocyte antigen receptors." in: Cell, Vol. 76, Issue 2, pp. 263-74, 1994 (PubMed).

Kinnaert, Pradier, Bournonville et al.: "Role of CD18-dependent and CD18-independent mechanisms in the increased leukocyte adhesiveness and in the variations of circulating white blood cell populations induced by anti-CD3 monoclonal antibodies." in: Transplant international : official journal of the European Society for Organ Transplantation, Vol. 9, Issue 4, pp. 386-91, 1997 (PubMed).

Han, Murray-Segal, Gershenzon et al.: "Idarubicin-145-2C11-F(ab')2 promotes peripheral tolerance and reduces chronic vascular disease in mouse cardiac allografts." in: Transplant immunology, Vol. 7, Issue 4, pp. 207-13, 2000 (PubMed).

Ko, Ko, Chang et al.: "alpha-Galactosylceramide can act as a nasal vaccine adjuvant inducing protective immune responses against viral infection and tumor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 5, pp. 3309-17, 2005 (PubMed).

Terrazas, Montero, Terrazas et al.: "Role of the programmed Death-1 pathway in the suppressive activity of alternatively activated macrophages in experimental cysticercosis." in: International journal for parasitology, Vol. 35, Issue 13, pp. 1349-58, 2005 (PubMed).

Podd, Thoits, Whitley et al.: "T cells in cryptopatch aggregates share TCR gamma variable region junctional sequences with gamma delta T cells in the small intestinal epithelium of mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 11, pp. 6532-42, 2006 (PubMed).

Tilley, Jaradat, Stapleton et al.: "Retinoid-related orphan receptor gamma controls immunoglobulin production and Th1/Th2 cytokine balance in the adaptive immune response to allergen." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 5, pp. 3208-18, 2007 (PubMed).

Wang, Huang, Goetzl: "Type 1 sphingosine 1-phosphate G protein-coupled receptor (S1P1) mediation of enhanced IL-4 generation by CD4 T cells from S1P1 transgenic mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 8, pp. 4885-90, 2007 (PubMed).

Xiao, Najafian, Reddy et al.: "Differential engagement of Tim-1 during activation can positively or negatively costimulate T cell expansion and effector function." in: The Journal of experimental medicine, Vol. 204, Issue 7, pp. 1691-702, 2007 (PubMed).