CD3 (CD3E) Antikörper

Antigen: CD3 (CD3E)

Synonyme: T3E, TCRE, FLJ18683

Klonalität: Monoklonal (17A2)

Wirt: Ratte

Reaktivität: Maus

Applikation: Durchflusszytometrie (FACS), Immunhistochemie (Gefrierschnitte) (IHC (fro)), Immunpräzipitation (IP), Aktivierung (Act), Abreicherung (Depletion)

Produktnummer: ABIN155329

Produktbeschreibung

Weitere Bezeichnung: CD3

Immunogen: gammadeltaTCR-positive T-T hybridoma D1

Format: Low endotoxin (sterile), Low-endotoxin, azide-free (LEAF)

Isotyp: IgG2b, kappa chain  (Passende Sekundärantikörper)

Klon: 17A2

Beschreibung: CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3epsilon, delta, gamma and zeta chains. It forms a TCR complex by associating with TCR alpha/beta or gamma/delta chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex. The 17A2 antibody recognizes epsilon/gamma (but not epsilon/delta) of CD3 complex. The 17A2 antibody can induce T cell activation and has been reported to deplete CD3 cells in vivo. Distribution: Thymocytes (differentiation dependent), mature T cells, NK-T cells Function: Antigen recognition, TCR signal transduction, T cell activation Synonyms: T cell antigen receptor complex, T3 Structure: Ig superfamily, CD3/TCR, 20 kD

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: immunoprecipitation, complement-mediated cytotoxicity, immunohistochemical staining of acetone-fixed frozen sections, in vitro stimulation of T cells and depletion of CD3 cells in vivo. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN155329). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Reinheit: LEAF

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.

Lagerung: The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.

Forschungsgebiet: CD Antigene

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

C57BL/6 mouse splenocytes stained with LEAF(TM) purified 17A2, followed by anti-rat IgG FITC

Publikationen

Publikationen: Mysliwietz, Thierfelder: "Antilymphocytic antibodies and marrow transplantation. XII. Suppression of graft-versus-host disease by T-cell-modulating and depleting antimouse CD3 antibody is most effective when preinjected in the marrow recipient." in: Blood, Vol. 80, Issue 10, pp. 2661-7, 1992 (PubMed).

Wu, Antica, Johnson et al.: "Developmental potential of the earliest precursor cells from the adult mouse thymus." in: The Journal of experimental medicine, Vol. 174, Issue 6, pp. 1617-27, 1992 (PubMed).

Davis: "T cell receptor gene diversity and selection." in: Annual review of biochemistry, Vol. 59, pp. 475-96, 1990 (PubMed).

Miescher, Schreyer, MacDonald: "Production and characterization of a rat monoclonal antibody against the murine CD3 molecular complex." in: Immunology letters, Vol. 23, Issue 2, pp. 113-8, 1990 (PubMed).

Weiss, Littman: "Signal transduction by lymphocyte antigen receptors." in: Cell, Vol. 76, Issue 2, pp. 263-74, 1994 (PubMed).

Zhang, McCormick, Desai et al.: "Murine sclerodermatous graft-versus-host disease, a model for human scleroderma: cutaneous cytokines, chemokines, and immune cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 6, pp. 3088-98, 2002 (PubMed).

Zan, Shima, Xu et al.: "The translesion DNA polymerase theta plays a dominant role in immunoglobulin gene somatic hypermutation." in: The EMBO journal, Vol. 24, Issue 21, pp. 3757-69, 2005 (PubMed).