CD2 Molecule (CD2) Antikörper

Antigen: CD2 Molecule (CD2)

Synonyme: T11, SRBC, FLJ46032, CD2, MGC140284, CD2R, LFA2, OX34, LFA-2, OX-34, Ly37, Ly-37

Klonalität: Monoklonal (RM2-5)

Wirt: Ratte

Reaktivität: Maus

Applikation: Durchflusszytometrie (FACS), Immunpräzipitation (IP), Blockierende Antikörper (Inhibition), Kostimulation

Produktnummer: ABIN155321

Produktbeschreibung

Weitere Bezeichnung: CD2

Immunogen: BALB/c mouse thymocytes

Format: Low endotoxin (sterile), Low-endotoxin, azide-free (LEAF)

Isotyp: IgG2b, lambda chain  (Passende Sekundärantikörper)

Klon: RM2-5

Beschreibung: CD2 is a 45-58 kD type I transmembrane glycoprotein, also known as LFA-2, T11 or Ly-37. It is a member of the Ig superfamily. Mouse CD2 is primarily expressed on T cells, B cells, thymocytes and NK cells. It is a ligand for CD48 and is involved in T cell activation and differentiation. The RM2-5 antibody can block CD2-mediated cell-cell adhesion. Distribution: T cells, thymocytes, NK cells, B cells Function: T cell activation, adhesion Synonyms: LFA-2, T11, Ly-37, SRBC-R Structure: Ig superfamily, 45-58 kD

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: in vitro blocking of CD2-mediated cell-cell adhesion and inhibition of T cell activation, blocking of T cell A.I.C.D., immunoprecipitation, and co-induction of thymocyte maturation. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN155321). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.25 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Reinheit: LEAF

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.

Lagerung: The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.

Forschungsgebiet: CD Antigene

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

C57BL/6 mouse splenocytes stained with LEAF(TM) purified RM2-5, followed by anti-rat IgG FITC

Publikationen

Publikationen: Nakamura, Takahashi, Fukazawa et al.: "Relative contribution of CD2 and LFA-1 to murine T and natural killer cell functions." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 145, Issue 11, pp. 3628-34, 1991 (PubMed).

Bierer, Sleckman, Ratnofsky et al.: "The biologic roles of CD2, CD4, and CD8 in T-cell activation." in: Annual review of immunology, Vol. 7, pp. 579-99, 1989 (PubMed).

Criado, Feito, Rojo: "CD4-dependent and -independent association of protein tyrosine kinases to the T cell receptor/CD3 complex of CD4+ mouse T lymphocytes." in: European journal of immunology, Vol. 26, Issue 6, pp. 1228-34, 1996 (PubMed).

Davis, van der Merwe: "The structure and ligand interactions of CD2: implications for T-cell function." in: Immunology today, Vol. 17, Issue 4, pp. 177-87, 1997 (PubMed).

Cibotti, Punt, Dash et al.: "Surface molecules that drive T cell development in vitro in the absence of thymic epithelium and in the absence of lineage-specific signals." in: Immunity, Vol. 6, Issue 3, pp. 245-55, 1997 (PubMed).

Ayroldi, Migliorati, Cannarile et al.: "CD2 rescues T cells from T-cell receptor/CD3 apoptosis: a role for the Fas/Fas-L system." in: Blood, Vol. 89, Issue 10, pp. 3717-26, 1997 (PubMed).