GFP Antikörper
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| Klonalität |
Polyklonal |
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Wirt
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Applikation
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 Alternativen Immunhistochemie (Gefrierschnitte) (IHC (fro)), Enzyme Immunoassay (EIA), Western Blot (WB)
Western Blot (WB) (209), Immunfluoreszenz (IF) (91), ELISA (88), Immunpräzipitation (IP) (88), Durchflusszytometrie (FACS) (64), Enzyme Immunoassay (EIA) (52), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (49), Immunhistochemie (IHC) (26), Immunhistochemie (Paraffinschnitte) (IHC (p)) (24), Immunzytochemie (ICC) (12), Dot Blot (Dot) (8), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (6), Intrazelluläre Durchflusszytometrie (ICFC) (4), Immunoelectron Microscopy (IEM) (3), To be determined by user (TBD) (2), Immunelektronenmikroskopie (EM) (1)
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| Produktnummer |
ABIN153484 |
| Menge |
0.1 mg (1.9 mg/ml) (Varianten) |
| Preis |
230,00 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 7 bis 10 Werktagen |
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Immunogen
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The immunogen is a GST-Green Fluorescent Protein (GFP) fusion proteincorresponding to the full length amino acid sequence (246aa) derived from the jellyfishAequorea victoria.
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Isotyp
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IgG2a (Passende Sekundärantikörper)
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Beschreibung
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Green fluorescence protein (GFP) is a 27 KDa protein derived from the jellyfish Aquoreavictoria, which emits green light (emission peak at a wavelenth of 509 nm) when excitedby blue light (excitation peak at a wavelenth of 395 nm). GFP has become an invaluabletool in cell biology research, since its intrinsic fluorescence can be visualized in livingcells. GFP fluorescence is stable under fixation conditions and suitable for a variety ofapplications. GFP has been widely used as a reporter for gene expression, enablingresearchers to visualize and localize GFP-tagged proteins within living cells without theneed for chemical staining. Other applications of GFP include assessment of proteinprotein interactions through the yeast two hybrid system and measurement of distancebetween proteins through fluorescence energy transfer (FRET) protocols. GFPtechnnology has considerably contributed to a greater understanding of cellularphysiology. Alternate Names: anti-Green Fluorescent Protein antibody.
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Spezifität
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Reactivity is observed against wild type and recombinant forms of GFP. No reaction isseen against RFP. Species Reactivity: All Species. Theoretical Molecular Weight: 27 kDa.
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Applikationshinweise
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Suggested working dilutions: ELISA 1:12000-1:60000,immunohistochemistry 1:1000-1:5000,Western Blot 1:3000-1:15000
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Konzentration
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1.9 mg/ml
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Buffer
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0.02M Potassium phosphate, 0.15M sodium chloride, [pH7.2]. Preservative: 0.1% sodium azide.
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Lagerung
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Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.
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Forschungsgebiet
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Tags/Labels
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Publikationen
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Sampson, Leyland, Dart: "Direct interaction between the actin-binding protein filamin-A and the inwardly rectifying potassium channel, Kir2.1." in: The Journal of biological chemistry, Vol. 278, Issue 43, pp. 41988-97, 2003 (PubMed).
Malagelada, Jin, Greene: "RTP801 is induced in Parkinson's disease and mediates neuron death by inhibiting Akt phosphorylation/activation." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 53, pp. 14363-71, 2009 (PubMed).
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Alternativen zu Antigen "GFP", Typ "Antikörper" finden
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Wirte
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Kaninchen (139), Maus (83), Ziege (46), Huhn (16), Ratte (3), Schaf (3)
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Reaktivitäten
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Aequorea victoria (81), Human (28), Maus (15), Fusion Protein (Tag) (14), Ratte (Rattus) (13), Alle Spezies (5), Rind (Kuh) (5), Hund (5), Kaninchen (4), Huhn (3), Qualle (1)
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Applikationen
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Western Blot (WB) (209), Immunfluoreszenz (IF) (91), ELISA (88), Immunpräzipitation (IP) (88), Durchflusszytometrie (FACS) (64), Enzyme Immunoassay (EIA) (52), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (49), Immunhistochemie (IHC) (26), Immunhistochemie (Paraffinschnitte) (IHC (p)) (24), Immunzytochemie (ICC) (12), Dot Blot (Dot) (8), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (6), Intrazelluläre Durchflusszytometrie (ICFC) (4), Immunoelectron Microscopy (IEM) (3), To be determined by user (TBD) (2), Immunelektronenmikroskopie (EM) (1)
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Konjugate
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HRP (21), Biotin (20), FITC (13), Alkaline Phosphatase (AP) (6), Alexa Fluor 647 (5), Texas Red (TR) (4), Alexa Flour 488 (3), Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), Cy3 (3), Cy5 (3), Cy5.5 (3), Cy7 (3), Gold (3), PE (3), PE,Cy3 (3), PE,Cy5 (3), PE,Cy5.5 (3), PE,Cy7 (3), RBITC (3), Agarose Beads (2), Rhodamine (1), TRITC (1)
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Epitope
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N-Term (4)
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