Collagen, Type I, alpha 1 (COL1A1) Antikörper

Antigen: Collagen, Type I, alpha 1 (COL1A1)

Synonyme: OI4, Cola1, Mov13, Cola-1, Mov-13, Col1a-1, COLIA1, MGC128258, col1a1, MGC52532, COL1A1, cb21, sb:cb21, sb:cb384, alpha1(I), wu:fa99c12, wu:fb02c06

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Säugetiere

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Enzyme Immunoassay (EIA), Western Blot (WB)

Produktnummer: ABIN153322

Produktbeschreibung

Weitere Bezeichnung: Collagen type I

Gen-ID: 1277, 29393

Immunogen: Collagen Type I from human and bovine placenta by limited pepsin digestion andselective salt precipitation.

Beschreibung: Collagens are highly conserved throughout evolution and are characterized by anuninterrupted Glycine-X-Y triplet repeat that is a necessary part of the triple helicalstructure. For these reasons it is often extremely difficult to generate antibodies withspecificities to collagens. The development of type specific antibodies is dependent onNON-DENATURED three-dimensional epitopes. This preparation results in a nativeconformation of the protein. Alternate Names: Anti-COL1A1 antibody, Anti-Collagen Of Skin Tendon And Bone antibody, Anti-CollagenType 1 antibody, Anti-OI4 antibody, Anti- Osteogenesis Imperfecta Type IV antibody,Anti-Pro alpha 1(I) collagen antibody.

Spezifität: Typically less than 1% cross reactivity against other types of collagens was detected byELISA against purified standards. This antibody reacts with most mammalian Type Icollagens. Minimum Cross Reactivity to Type II, III, IV, V and VI Collagens. Species Reactivity: Reacts with human and rat. Expected to react with most mammalian Type I collagens. Localization: Extracellular matrix.

Anwendungen

Applikationshinweise: Suggested working dilutions: ELISA - 1:5,000-1:50,000,Western Blot - 1:5,000-1:50,000,Immunohistochemistry-Frozen - 1:50-1:200

Konzentration: 1.17 mg/ml

Buffer: 0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA [pH8]. Preservative: 0.01% sodium azide.

Lagerung: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.

Forschungsgebiet: Extrazelluläre Matrix

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-Collagen, Type I, alpha 1 (COL1A1) antibody

Publikationen

Publikationen: Stefanovic, Schnabl, Brenner: "Inhibition of collagen alpha 1(I) expression by the 5' stem-loop as a molecular decoy." in: The Journal of biological chemistry, Vol. 277, Issue 20, pp. 18229-37, 2002 (PubMed).

Hazra, Xiong, Wang et al.: "Peroxisome proliferator-activated receptor gamma induces a phenotypic switch from activated to quiescent hepatic stellate cells." in: The Journal of biological chemistry, Vol. 279, Issue 12, pp. 11392-401, 2004 (PubMed).

Hashimoto, Jin, Liu et al.: "Bone marrow-derived progenitor cells in pulmonary fibrosis." in: The Journal of clinical investigation, Vol. 113, Issue 2, pp. 243-52, 2004 (PubMed).

She, Xiong, Hazra et al.: "Adipogenic transcriptional regulation of hepatic stellate cells." in: The Journal of biological chemistry, Vol. 280, Issue 6, pp. 4959-67, 2005 (PubMed).

Keophiphath, Achard, Henegar et al.: "Macrophage-secreted factors promote a profibrotic phenotype in human preadipocytes." in: Molecular endocrinology (Baltimore, Md.), Vol. 23, Issue 1, pp. 11-24, 2009 (PubMed).