PR Domain Containing 1, with ZNF Domain (PRDM1) Antikörper

Antigen: PR Domain Containing 1, with ZNF Domain (PRDM1)

Synonyme: BLIMP1, PRDI-BF1, MGC118922, MGC118923, MGC118924, MGC118925, GB11750, blimp-1, CG5249, dBlimp-1, dBlimp1, DmelCG5249, nrd, ubo, prdm1, Blimp1, Blimp-1, MGC91885, zgc:91885, wu:fb99c12, ZNFPR1A1, MGC52536, PRDM1, blimp1, xblimp1, prdi-bf1

Klonalität: Monoklonal (3H2-E8)

Wirt: Maus

Reaktivität: Human, Maus

Applikation: Enzyme Immunoassay (EIA), Immunfluoreszenz (IF), Immunpräzipitation (IP), Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB)

Produktnummer: ABIN153175

Produktbeschreibung

Weitere Bezeichnung: PRDM1 / BLIMP1

Gen-ID: 639

Immunogen: A fragment of the mouse Blimp-1 (residues 199-409).

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: 3H2-E8

Beschreibung: Blimp-1 (B lymphocyte maturation protein-1) is a nuclear protein with five zinc fingers inits carboxy terminus. It is a transcriptional repressor that is both required and sufficientto trigger terminal differentiation of B lymphocytes and monocyte/macrophages. Blimp-1is expressed in, and may be required for terminally differentiated epithelial cells, retinalneurons and other lineage cells. It has also been implicated in the repression ofbeta-interferon (IFN-beta) and c-myc gene expression. Alternate Names: anti-BLIMP 1 antibody, anti-B Lymphocyte Induced Maturation Protein 1 antibody,anti-BLIMP1 antibody, anti-Positive Regulatory Domain I Binding Factor 1 antibody,anti-PR Domain Containing 1 antibody, anti-PRDI BF1 antibody, anti-PRDM1 antibody.

Spezifität: This antibody is specific for the Blimp-1. Species Reactivity: Mouse and human (see Specific Reference). Other species have not been tested. Localization: Nuclear.

Synonyme: BLIMP1, PRDI-BF1, MGC118922, MGC118923, MGC118924, MGC118925, GB11750, blimp-1, CG5249, dBlimp-1, dBlimp1, DmelCG5249, nrd, ubo, prdm1, Blimp1, Blimp-1, MGC91885, zgc:91885, wu:fb99c12, ZNFPR1A1, MGC52536, PRDM1, blimp1, xblimp1, prdi-bf1

Anwendungen

Applikationshinweise: Suggested working dilutions: Western Blot 1:1000-1:5000,Positive Controls: P3X (murine plasmocytoma) cell lysates

Konzentration: 1.2 mg/ml

Buffer: 0.025 ml of Protein G purified mouse ascites [1.2 mg/ml] in PBS containing 0.1%Sodium Azide.

Lagerung: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Detection of Blimp-1 in murine plasmacytoma cell lysate (P3X) using ABIN153174. 1881: murine pre-B cell lysate (negative control). Photo courtesy of DA Savitsky, Columbia University.

Double IF for Blimp-1 (green) and Ki-67 (proliferation, red) with DAPI counterstain, at 10x magnification (scale bar is 10µm). Positive surface epithelium and centrocytes are labelled. Photo courtesy of Dr. Giorgio Cattoretti, Institute for Cancer Genetics, Columbia University, NY.

Publikationen

Publikationen: Lin, Wong, Calame: "Repression of c-myc transcription by Blimp-1, an inducer of terminal B cell differentiation." in: Science (New York, N.Y.), Vol. 276, Issue 5312, pp. 596-9, 1997 (PubMed).

Angelin-Duclos, Cattoretti, Lin et al.: "Commitment of B lymphocytes to a plasma cell fate is associated with Blimp-1 expression in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 10, pp. 5462-71, 2000 (PubMed).

Lin, Angelin-Duclos, Kuo et al.: "Blimp-1-dependent repression of Pax-5 is required for differentiation of B cells to immunoglobulin M-secreting plasma cells." in: Molecular and cellular biology, Vol. 22, Issue 13, pp. 4771-80, 2002 (PubMed).

Chang, Cattoretti, Calame: "The dynamic expression pattern of B lymphocyte induced maturation protein-1 (Blimp-1) during mouse embryonic development." in: Mechanisms of development, Vol. 117, Issue 1-2, pp. 305-9, 2002 (PubMed).

Ohinata, Payer, OCarroll et al.: "Blimp1 is a critical determinant of the germ cell lineage in mice." in: Nature, Vol. 436, Issue 7048, pp. 207-13, 2005 (PubMed).

Severa, Coccia, Fitzgerald: "Toll-like receptor-dependent and -independent viperin gene expression and counter-regulation by PRDI-binding factor-1/BLIMP1." in: The Journal of biological chemistry, Vol. 281, Issue 36, pp. 26188-95, 2006 (PubMed).

Liu, Leboeuf, Shi et al.: "Rituximab plus CHOP (R-CHOP) overcomes PRDM1-associated resistance to chemotherapy in patients with diffuse large B-cell lymphoma." in: Blood, Vol. 110, Issue 1, pp. 339-44, 2007 (PubMed).

John, Clements, Russell et al.: "Ets-1 regulates plasma cell differentiation by interfering with the activity of the transcription factor Blimp-1." in: The Journal of biological chemistry, Vol. 283, Issue 2, pp. 951-62, 2008 (PubMed).